The first molecular detection of Aichi virus 1 in raw sewage and mussels collected in South Africa

Oikwathaile Onosi; Nicole S. Upfold; Michael D. Jukes; Garry A. Luke; Caroline Knox

doi:10.1007/s12560-018-9362-4

The first molecular detection of Aichi virus 1 in raw sewage and mussels collected in South Africa

Oikwathaile Onosi, Nicole S. Upfold, Michael D. Jukes, Garry A. Luke, Caroline Knox

Research output: Contribution to journal › Article › peer-review

Abstract

Aichi virus 1 (AiV-1) has a worldwide distribution and is associated with gastroenteritis in humans. In this study, raw sewage and mussel samples were analyzed for the presence of AiV-1 using reverse transcription-PCR (RT-PCR). Amplification and sequencing of the 3CD and VP1 genomic regions followed by phylogenetic analysis using selected genome sequences revealed the presence of AiV-1, genotype B. The results highlight the importance of further screening to evaluate the prevalence and epidemiology of this clinically important virus in South Africa.

Original language	English
Pages (from-to)	96-100
Number of pages	5
Journal	Food and Environmental Virology
Volume	11
Issue number	1
Early online date	17 Dec 2018
DOIs	https://doi.org/10.1007/s12560-018-9362-4
Publication status	Published - 2019

Keywords

Aichi virus 1
Kobuvirus
Mussels
Waste water
Picornaviridae

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title = "The first molecular detection of Aichi virus 1 in raw sewage and mussels collected in South Africa",

abstract = "Aichi virus 1 (AiV-1) has a worldwide distribution and is associated with gastroenteritis in humans. In this study, raw sewage and mussel samples were analyzed for the presence of AiV-1 using reverse transcription-PCR (RT-PCR). Amplification and sequencing of the 3CD and VP1 genomic regions followed by phylogenetic analysis using selected genome sequences revealed the presence of AiV-1, genotype B. The results highlight the importance of further screening to evaluate the prevalence and epidemiology of this clinically important virus in South Africa.",

keywords = "Aichi virus 1, Kobuvirus, Mussels, Waste water, Picornaviridae",

author = "Oikwathaile Onosi and Upfold, {Nicole S.} and Jukes, {Michael D.} and Luke, {Garry A.} and Caroline Knox",

note = "This work was supported by Medical Research Council (MRC, South Africa) and Research Council (RC, Rhodes University) grants. OO was supported by the Department of Public Health, Ministry of Health and wellness, Botswana. NU was supported by postgraduate fellowships from the National Research Foundation of South Africa and the German Academic Exchange Service (DAAD). ",

year = "2019",

doi = "10.1007/s12560-018-9362-4",

language = "English",

volume = "11",

pages = "96--100",

journal = "Food and Environmental Virology",

issn = "1867-0342",

publisher = "Springer",

number = "1",

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TY - JOUR

T1 - The first molecular detection of Aichi virus 1 in raw sewage and mussels collected in South Africa

AU - Onosi, Oikwathaile

AU - Upfold, Nicole S.

AU - Jukes, Michael D.

AU - Luke, Garry A.

AU - Knox, Caroline

N1 - This work was supported by Medical Research Council (MRC, South Africa) and Research Council (RC, Rhodes University) grants. OO was supported by the Department of Public Health, Ministry of Health and wellness, Botswana. NU was supported by postgraduate fellowships from the National Research Foundation of South Africa and the German Academic Exchange Service (DAAD).

PY - 2019

Y1 - 2019

N2 - Aichi virus 1 (AiV-1) has a worldwide distribution and is associated with gastroenteritis in humans. In this study, raw sewage and mussel samples were analyzed for the presence of AiV-1 using reverse transcription-PCR (RT-PCR). Amplification and sequencing of the 3CD and VP1 genomic regions followed by phylogenetic analysis using selected genome sequences revealed the presence of AiV-1, genotype B. The results highlight the importance of further screening to evaluate the prevalence and epidemiology of this clinically important virus in South Africa.

AB - Aichi virus 1 (AiV-1) has a worldwide distribution and is associated with gastroenteritis in humans. In this study, raw sewage and mussel samples were analyzed for the presence of AiV-1 using reverse transcription-PCR (RT-PCR). Amplification and sequencing of the 3CD and VP1 genomic regions followed by phylogenetic analysis using selected genome sequences revealed the presence of AiV-1, genotype B. The results highlight the importance of further screening to evaluate the prevalence and epidemiology of this clinically important virus in South Africa.

KW - Aichi virus 1

KW - Kobuvirus

KW - Mussels

KW - Waste water

KW - Picornaviridae

U2 - 10.1007/s12560-018-9362-4

DO - 10.1007/s12560-018-9362-4

M3 - Article

SN - 1867-0342

VL - 11

SP - 96

EP - 100

JO - Food and Environmental Virology

JF - Food and Environmental Virology

IS - 1

ER -

The first molecular detection of Aichi virus 1 in raw sewage and mussels collected in South Africa

Abstract

Keywords

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