The first enzymatic method for C-F-18 bond formation: the synthesis of 5 '-[F-18]-fluoro-5 ' deoxyadenosine for imaging with PET

L Martarello, C Schaffrath, H Deng, AD Gee, A Lockhart, David O'Hagan

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43 Citations (Scopus)

Abstract

The use of the key enzyme involved in carbon-fluorine bond formation in Streptomyces cattleya catalysing the formation of 5'-fluoro-5'-deoxyadenosine (5'-FDA) from fluoride ion and S-adenosyl-L-methionine (SAM) was explored for its potential application in fluorine-18 labelling of the adenosine derivative. Enzymatic radiolabelling of [F-18]-5'-FDA was successfully carried out starting from SAM and [F-18]HF when the concentration of the enzyme preparation was increased from sub-mg/ml values to mg/ml values. The purity of the enzyme had no measurable effect on the radiochemical yield of the reaction and the radiochemical purity of [F-18]-5'-FDA. Copyright (C) 2003 John Wiley Sons, Ltd.

Original languageEnglish
Pages (from-to)1181
Number of pages1181
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume46
DOIs
Publication statusPublished - Nov 2003

Keywords

  • fluorinase
  • enzymatic radiolabelling
  • fluorine-18
  • [F-18]-5 '-FDA

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