The Schizosaccharomyces pombe rfc3+ gene encodes a homologue of the human hRFC36 and Saccharomyces cerevisiae Rfc3 subunits of replication factor C

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18 Citations (Scopus)

Abstract

In the yeasts Saccharomyces cevevisiae and Schizosaccharomyces pombe replication factor C (RF-C) plays key roles both in chromosomal DNA replication and in DNA replication checkpoint function. At the replication fork, the five-subunit RF-C complex functions to load the trimeric polymerase accessory factor PCNA onto DNA. PCNA then acts as a sliding clamp tethering Pol delta to the DNA to maximise its processivity. Here we describe the cloning of the S. pombe rfc3(+) gene, encoding a homologue of the S. cevevisiae Rfc3 and human hRFC36 proteins. The 1026 bp rfc3(+) ORF is interrupted by five introns, ranging in size from 49 to 165 bp. The spliced ORF is predicted to encode a 342 amino-acid protein that is approximately 50% identical at the amino acid sequence level to the S. cevevisiae Rfc3 and human hRFC36 proteins. As expected, S. pombe rfc3(+) is an essential gene, with rfc3 Delta cells being defective for DNA replication. Loss of rfc3(+) function can be rescued by heterologous expression of either the S. cerevisiae Rfc3 or human hRFC36 proteins in S. pombe.

Original languageEnglish
Pages (from-to)159-167
Number of pages9
JournalCurrent Genetics
Volume37
Issue number3
DOIs
Publication statusPublished - Mar 2000

Keywords

  • DNA replication
  • replication factor C
  • fission yeast
  • SV40 DNA-REPLICATION
  • S-PHASE CHECKPOINT
  • FISSION YEAST
  • IDENTIFICATION
  • COMPLEX
  • CLONING
  • RECONSTITUTION
  • COMPONENT
  • PROTEINS
  • CELLS

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