The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation

Aipo Diao, Dinah Rahman, Darryl J C Pappin, John Lucocq, Martin Lowe

Research output: Contribution to journalArticlepeer-review

187 Citations (Scopus)

Abstract

Fragmentation of the mammalian Golgi apparatus during mitosis requires the phosphorylation of a specific subset of Golgi-associated proteins. We have used a biochemical approach to characterize these proteins and report here the identification of golgin-84 as a novel mitotic target. Using cryoelectron microscopy we could localize golgin-84 to the cis-Golgi network and found that it is enriched on tubules emanating from the lateral edges of, and often connecting, Golgi stacks. Golgin-84 binds to active rab1 but not cis-Golgi matrix proteins. Overexpression or depletion of golgin-84 results in fragmentation of the Golgi ribbon. Strikingly, the Golgi ribbon is converted into mini-stacks constituting only approximately 25% of the volume of a normal Golgi apparatus upon golgin-84 depletion. These mini-stacks are able to carry out protein transport, though with reduced efficiency compared with a normal Golgi apparatus. Our results suggest that golgin-84 plays a key role in the assembly and maintenance of the Golgi ribbon in mammalian cells.
Original languageEnglish
Pages (from-to)201-12
Number of pages12
JournalJournal of Cell Biology
Volume160
Issue number2
DOIs
Publication statusPublished - 20 Jan 2003

Keywords

  • Animals
  • Autoantigens
  • Endoplasmic Reticulum
  • Eukaryotic Cells
  • Gene Expression Regulation
  • Golgi Apparatus
  • HeLa Cells
  • Humans
  • Intracellular Membranes
  • Membrane Proteins
  • Microscopy, Electron
  • Microtubule Proteins
  • Mitosis
  • Phosphoproteins
  • Protein Transport
  • RNA, Small Interfering
  • Rats
  • Recombinant Fusion Proteins
  • Subcellular Fractions
  • Viral Proteins
  • rab1 GTP-Binding Proteins

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