The Analysis of Intact Glucosinolates by MALDI-TOF Mass Spectrometry

Catherine Helen Botting; NE Davidson; DW Griffiths; RN Bennett; Nigel Peter Botting

doi:10.1021/jf011129a

The Analysis of Intact Glucosinolates by MALDI-TOF Mass Spectrometry

Catherine Helen Botting, NE Davidson, DW Griffiths, RN Bennett, Nigel Peter Botting

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Glucosinolates are naturally occurring plant compounds that may be important in the dietary prevention of cancer. This study shows that they can be detected in their intact form by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with a high degree of sensitivity. The methodology was used to characterize a number of individual glucosinolates either produced by synthetic chemistry or isolated from plants. The method was used for crude plant extracts to rapidly examine the glucosinolate profile of the plant. The results for a range of plant extracts showed good agreement with previous LC-MS analysis of the desulfoglucosinolates from the same samples.

Original language	English
Pages (from-to)	983-988
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	50
DOIs	https://doi.org/10.1021/jf011129a
Publication status	Published - 27 Feb 2002

Keywords

glucosinolates
Brassica sp.
MALDI-TOF
metabolornics
LIQUID-CHROMATOGRAPHY
LASER-DESORPTION
MATRIX
IDENTIFICATION
PLANTS
FOOD
MS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/jf011129a

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title = "The Analysis of Intact Glucosinolates by MALDI-TOF Mass Spectrometry",

abstract = "Glucosinolates are naturally occurring plant compounds that may be important in the dietary prevention of cancer. This study shows that they can be detected in their intact form by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with a high degree of sensitivity. The methodology was used to characterize a number of individual glucosinolates either produced by synthetic chemistry or isolated from plants. The method was used for crude plant extracts to rapidly examine the glucosinolate profile of the plant. The results for a range of plant extracts showed good agreement with previous LC-MS analysis of the desulfoglucosinolates from the same samples.",

keywords = "glucosinolates, Brassica sp., MALDI-TOF, metabolornics, LIQUID-CHROMATOGRAPHY, LASER-DESORPTION, MATRIX, IDENTIFICATION, PLANTS, FOOD, MS",

author = "Botting, {Catherine Helen} and NE Davidson and DW Griffiths and RN Bennett and Botting, {Nigel Peter}",

year = "2002",

month = feb,

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doi = "10.1021/jf011129a",

language = "English",

volume = "50",

pages = "983--988",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society (ACS)",

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TY - JOUR

T1 - The Analysis of Intact Glucosinolates by MALDI-TOF Mass Spectrometry

AU - Botting, Catherine Helen

AU - Davidson, NE

AU - Griffiths, DW

AU - Bennett, RN

AU - Botting, Nigel Peter

PY - 2002/2/27

Y1 - 2002/2/27

N2 - Glucosinolates are naturally occurring plant compounds that may be important in the dietary prevention of cancer. This study shows that they can be detected in their intact form by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with a high degree of sensitivity. The methodology was used to characterize a number of individual glucosinolates either produced by synthetic chemistry or isolated from plants. The method was used for crude plant extracts to rapidly examine the glucosinolate profile of the plant. The results for a range of plant extracts showed good agreement with previous LC-MS analysis of the desulfoglucosinolates from the same samples.

AB - Glucosinolates are naturally occurring plant compounds that may be important in the dietary prevention of cancer. This study shows that they can be detected in their intact form by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with a high degree of sensitivity. The methodology was used to characterize a number of individual glucosinolates either produced by synthetic chemistry or isolated from plants. The method was used for crude plant extracts to rapidly examine the glucosinolate profile of the plant. The results for a range of plant extracts showed good agreement with previous LC-MS analysis of the desulfoglucosinolates from the same samples.

KW - glucosinolates

KW - Brassica sp.

KW - MALDI-TOF

KW - metabolornics

KW - LIQUID-CHROMATOGRAPHY

KW - LASER-DESORPTION

KW - MATRIX

KW - IDENTIFICATION

KW - PLANTS

KW - FOOD

KW - MS

UR - http://www.scopus.com/inward/record.url?scp=0037180953&partnerID=8YFLogxK

UR - http://pubs.acs.org/cgi-bin/article.cgi/jafcau/2002/50/i05/pdf/jf011129a.pdf

U2 - 10.1021/jf011129a

DO - 10.1021/jf011129a

M3 - Article

SN - 0021-8561

VL - 50

SP - 983

EP - 988

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

ER -

The Analysis of Intact Glucosinolates by MALDI-TOF Mass Spectrometry

Abstract

Keywords

UN SDGs

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