The Analysis of Intact Glucosinolates by MALDI-TOF Mass Spectrometry

Catherine Helen Botting, NE Davidson, DW Griffiths, RN Bennett, Nigel Peter Botting

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Glucosinolates are naturally occurring plant compounds that may be important in the dietary prevention of cancer. This study shows that they can be detected in their intact form by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with a high degree of sensitivity. The methodology was used to characterize a number of individual glucosinolates either produced by synthetic chemistry or isolated from plants. The method was used for crude plant extracts to rapidly examine the glucosinolate profile of the plant. The results for a range of plant extracts showed good agreement with previous LC-MS analysis of the desulfoglucosinolates from the same samples.

Original languageEnglish
Pages (from-to)983-988
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume50
DOIs
Publication statusPublished - 27 Feb 2002

Keywords

  • glucosinolates
  • Brassica sp.
  • MALDI-TOF
  • metabolornics
  • LIQUID-CHROMATOGRAPHY
  • LASER-DESORPTION
  • MATRIX
  • IDENTIFICATION
  • PLANTS
  • FOOD
  • MS

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