Temporal dynamics of cytomegalovirus chromatin assembly in productively infected human cells

Alexandra Nitzsche, Christina Paulus, Michael Nevels

Research output: Contribution to journalArticlepeer-review

86 Citations (Scopus)

Abstract

The genomes of herpesviruses, including human cytomegalovirus (CMV), are double-stranded DNA molecules maintained as episomes during infection. The viral DNA lacks histones when encapsidated in the virion. However, it has been found histone associated inside infected cells, implying unidentified chromatin assembly mechanisms. Our results indicate that components of the host cell nucleosome deposition machinery target intranuclear CMV DNA, resulting in stepwise viral-chromatin assembly. CMV genomes undergo limited histone association and nucleosome assembly as early as 30 min after infection via DNA replication-independent mechanisms. Low average viral-genome chromatinization is maintained throughout the early stages of infection. The late phase of infection is characterized by a striking increase in average histone occupancy coupled with the process of viral-DNA replication. While the initial chromatinization affected all analyzed parts of the CMV chromosome, a subset of viral genomic regions, including the major immediate-early promoter, proved to be largely resistant to replication-dependent histone deposition. Finally, our results predict the likely requirement for an unanticipated chromatin disassembly process that enables packaging of histone-free DNA into progeny capsids.
Original languageEnglish
Pages (from-to)11167-80
Number of pages14
JournalJournal of Virology
Volume82
Issue number22
DOIs
Publication statusPublished - Nov 2008

Keywords

  • Cell Line
  • Chromatin Assembly and Disassembly
  • Cytomegalovirus
  • DNA, Viral
  • Histones
  • Humans

Fingerprint

Dive into the research topics of 'Temporal dynamics of cytomegalovirus chromatin assembly in productively infected human cells'. Together they form a unique fingerprint.

Cite this