Targeted optical injection of gold nanoparticles into single mammalian cells

Craig McDougall; David J. Stevenson; Christian Thomas Alcuin Brown; Francis James Gunn-Moore; Kishan Dholakia

doi:10.1002/jbio.200910030

Targeted optical injection of gold nanoparticles into single mammalian cells

Craig McDougall, David J. Stevenson, Christian Thomas Alcuin Brown, Francis James Gunn-Moore, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

We present an all optical technique for the targeted delivery of single 100 nm diameter gold nanoparticles into a specified region of the interior of an individual mammalian cell through a combination of optical tweezing and optical injection. The internalisation of the nanoparticle is verified by confocal laser scanning microscopy and confocal laser scanning reflectance microscopy. This represents the first time that nano sized particles have been tweezed and optically injected into mammalian cells using only light, and provides a novel methodology for internalising nanosphere based biosensors within specific intracellular regions of a mammalian cell.

Confocal cross sections in xy (main), xz (bottom) and yz (right) of a Chinese hamster ovary cell (green) containing an optically injected 100 nm gold nanoparticle (red) within the nucleus.

[GRAPHICS]

Original language	English
Pages (from-to)	736-743
Number of pages	8
Journal	Journal of Biophotonics
Volume	2
Issue number	12
DOIs	https://doi.org/10.1002/jbio.200910030
Publication status	Published - Dec 2009

Keywords

photoporation
optical tweezing
optical trapping
optical injection
TRANSFECTION
DNA
PARTICLES
INSERTION
SURFACE
FORCE

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10.1002/jbio.200910030

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title = "Targeted optical injection of gold nanoparticles into single mammalian cells",

abstract = "We present an all optical technique for the targeted delivery of single 100 nm diameter gold nanoparticles into a specified region of the interior of an individual mammalian cell through a combination of optical tweezing and optical injection. The internalisation of the nanoparticle is verified by confocal laser scanning microscopy and confocal laser scanning reflectance microscopy. This represents the first time that nano sized particles have been tweezed and optically injected into mammalian cells using only light, and provides a novel methodology for internalising nanosphere based biosensors within specific intracellular regions of a mammalian cell.Confocal cross sections in xy (main), xz (bottom) and yz (right) of a Chinese hamster ovary cell (green) containing an optically injected 100 nm gold nanoparticle (red) within the nucleus.[GRAPHICS](C) 2009 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

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AU - Stevenson, David J.

AU - Brown, Christian Thomas Alcuin

AU - Gunn-Moore, Francis James

AU - Dholakia, Kishan

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KW - FORCE

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Targeted optical injection of gold nanoparticles into single mammalian cells

Abstract

Keywords

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