Synthesis of Spin-Labeled RNAs for Long Range Distance Measurements by PELDOR

N. Piton; Olav Schiemann; Y. Mu; G. Stock; T.F. Prisner; J.W. Engels

doi:10.1081/NCN-200060139

Synthesis of Spin-Labeled RNAs for Long Range Distance Measurements by PELDOR

N. Piton, Olav Schiemann, Y. Mu, G. Stock, T.F. Prisner, J.W. Engels

Research output: Contribution to journal › Article › peer-review

Abstract

Site directed spin labeled RNA duplexes with different interspin distances were synthesized. The radical 2,2,5,5-tetramethyl-pyrrolin-1-yloxyl-3-acetylene (TPA) was introduced during the solid-phase synthesis through a Sonogashira cross-coupling with 5-iodo-uridine. T-m and CD studies showed that the spin label does not to disturb significantly the A-form of these duplexes. 4-Pulse Electron Double Resonance (PELDOR) was then used to measure intramolecular spin-spin distances of 19.3, 33.0 and 40.9 angstrom, which are in very good agreement with the calculated values of 17 6 32.1 and 39.1 angstrom, obtained from Molecular Dynamics (MD) simulations.

Original language	English
Pages (from-to)	771-775
Number of pages	5
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	24
Issue number	5-7
DOIs	https://doi.org/10.1081/NCN-200060139
Publication status	Published - Apr 2005

Keywords

RESONANCE SPECTROSCOPY EPR
TERTIARY STRUCTURE
DYNAMICS
SOLIDS
ECHO

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10.1081/NCN-200060139

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title = "Synthesis of Spin-Labeled RNAs for Long Range Distance Measurements by PELDOR",

abstract = "Site directed spin labeled RNA duplexes with different interspin distances were synthesized. The radical 2,2,5,5-tetramethyl-pyrrolin-1-yloxyl-3-acetylene (TPA) was introduced during the solid-phase synthesis through a Sonogashira cross-coupling with 5-iodo-uridine. T-m and CD studies showed that the spin label does not to disturb significantly the A-form of these duplexes. 4-Pulse Electron Double Resonance (PELDOR) was then used to measure intramolecular spin-spin distances of 19.3, 33.0 and 40.9 angstrom, which are in very good agreement with the calculated values of 17 6 32.1 and 39.1 angstrom, obtained from Molecular Dynamics (MD) simulations.",

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author = "N. Piton and Olav Schiemann and Y. Mu and G. Stock and T.F. Prisner and J.W. Engels",

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T1 - Synthesis of Spin-Labeled RNAs for Long Range Distance Measurements by PELDOR

AU - Piton, N.

AU - Schiemann, Olav

AU - Mu, Y.

AU - Stock, G.

AU - Prisner, T.F.

AU - Engels, J.W.

PY - 2005/4

Y1 - 2005/4

N2 - Site directed spin labeled RNA duplexes with different interspin distances were synthesized. The radical 2,2,5,5-tetramethyl-pyrrolin-1-yloxyl-3-acetylene (TPA) was introduced during the solid-phase synthesis through a Sonogashira cross-coupling with 5-iodo-uridine. T-m and CD studies showed that the spin label does not to disturb significantly the A-form of these duplexes. 4-Pulse Electron Double Resonance (PELDOR) was then used to measure intramolecular spin-spin distances of 19.3, 33.0 and 40.9 angstrom, which are in very good agreement with the calculated values of 17 6 32.1 and 39.1 angstrom, obtained from Molecular Dynamics (MD) simulations.

AB - Site directed spin labeled RNA duplexes with different interspin distances were synthesized. The radical 2,2,5,5-tetramethyl-pyrrolin-1-yloxyl-3-acetylene (TPA) was introduced during the solid-phase synthesis through a Sonogashira cross-coupling with 5-iodo-uridine. T-m and CD studies showed that the spin label does not to disturb significantly the A-form of these duplexes. 4-Pulse Electron Double Resonance (PELDOR) was then used to measure intramolecular spin-spin distances of 19.3, 33.0 and 40.9 angstrom, which are in very good agreement with the calculated values of 17 6 32.1 and 39.1 angstrom, obtained from Molecular Dynamics (MD) simulations.

KW - RESONANCE SPECTROSCOPY EPR

KW - TERTIARY STRUCTURE

KW - DYNAMICS

KW - SOLIDS

KW - ECHO

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DO - 10.1081/NCN-200060139

M3 - Article

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JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

IS - 5-7

ER -

Synthesis of Spin-Labeled RNAs for Long Range Distance Measurements by PELDOR

Abstract

Keywords

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