Synthesis of Spin-Labeled RNAs for Long Range Distance Measurements by PELDOR

N. Piton, Olav Schiemann, Y. Mu, G. Stock, T.F. Prisner, J.W. Engels

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Site directed spin labeled RNA duplexes with different interspin distances were synthesized. The radical 2,2,5,5-tetramethyl-pyrrolin-1-yloxyl-3-acetylene (TPA) was introduced during the solid-phase synthesis through a Sonogashira cross-coupling with 5-iodo-uridine. T-m and CD studies showed that the spin label does not to disturb significantly the A-form of these duplexes. 4-Pulse Electron Double Resonance (PELDOR) was then used to measure intramolecular spin-spin distances of 19.3, 33.0 and 40.9 angstrom, which are in very good agreement with the calculated values of 17 6 32.1 and 39.1 angstrom, obtained from Molecular Dynamics (MD) simulations.

Original languageEnglish
Pages (from-to)771-775
Number of pages5
JournalNucleosides, Nucleotides and Nucleic Acids
Volume24
Issue number5-7
DOIs
Publication statusPublished - Apr 2005

Keywords

  • RESONANCE SPECTROSCOPY EPR
  • TERTIARY STRUCTURE
  • DYNAMICS
  • SOLIDS
  • ECHO

Fingerprint

Dive into the research topics of 'Synthesis of Spin-Labeled RNAs for Long Range Distance Measurements by PELDOR'. Together they form a unique fingerprint.

Cite this