Synthesis of 6-dimethyl amino-9- [3'- (O-methyl)S) -[UL-14C] -tyrosinylamino) -3'-deoxy-b-D -ribofuranosyl] Purine.

In order to investigate and further refine the mechanism of the unique cleavage activity of the 18 amino acid 2A region of the foot-and-mouth-disease virus (FMDV), the synthesis of C-14-labelled puromycin is required. Puromycin is an inhibitor of protein synthesis and is an analogue of the terminal aminoacyl-adenosine portion of aminoacyl-tRNA. A short and expedient four step synthesis of 6-dimethylamino-9-[3'-(O-methyl) (2S)-[UL-C-14]tyrosinylamino)-3'-deoxy-beta-D-ribofuranosyl] purine (C-14-labelled puromycin) starting from (2S)-[UL-C-14]-tyrosine is therefore described.