Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups

J Grzybowski; DW Will; Richard Edward Randall; CA Smith; T Brown

doi:10.1093/nar/21.8.1705

Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups

J Grzybowski, DW Will, Richard Edward Randall, CA Smith, T Brown

Research output: Contribution to journal › Article › peer-review

Abstract

A series of non-nucleoslde-based 2,4-dlnltrophenyl (DNP) phosphoramidltes have been prepared and used in the multiple labelling of ollgonucleotldes during solid-phase synthesis. The length of spacer arm between the DNP label and the ollgonucleotlde phosphate backbone, and the number of attached DNP groups have both been varied In order to determine the optimum conditions for antl-DNP antibody binding. Detection using enzyme-linked colorimetrlc techniques showed sensitivity equivalent to that obtainable using biotinylated oligonucleotides.

Original language	English
Pages (from-to)	1705-1712
Journal	Nucleic Acids Research
Volume	21
Issue number	8
DOIs	https://doi.org/10.1093/nar/21.8.1705
Publication status	Published - 25 Apr 1993

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10.1093/nar/21.8.1705

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title = "Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups",

abstract = "A series of non-nucleoslde-based 2,4-dlnltrophenyl (DNP) phosphoramidltes have been prepared and used in the multiple labelling of ollgonucleotldes during solid-phase synthesis. The length of spacer arm between the DNP label and the ollgonucleotlde phosphate backbone, and the number of attached DNP groups have both been varied In order to determine the optimum conditions for antl-DNP antibody binding. Detection using enzyme-linked colorimetrlc techniques showed sensitivity equivalent to that obtainable using biotinylated oligonucleotides. ",

author = "J Grzybowski and DW Will and Randall, \{Richard Edward\} and CA Smith and T Brown",

year = "1993",

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doi = "10.1093/nar/21.8.1705",

language = "English",

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TY - JOUR

T1 - Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups

AU - Grzybowski, J

AU - Will, DW

AU - Randall, Richard Edward

AU - Smith, CA

AU - Brown, T

PY - 1993/4/25

Y1 - 1993/4/25

N2 - A series of non-nucleoslde-based 2,4-dlnltrophenyl (DNP) phosphoramidltes have been prepared and used in the multiple labelling of ollgonucleotldes during solid-phase synthesis. The length of spacer arm between the DNP label and the ollgonucleotlde phosphate backbone, and the number of attached DNP groups have both been varied In order to determine the optimum conditions for antl-DNP antibody binding. Detection using enzyme-linked colorimetrlc techniques showed sensitivity equivalent to that obtainable using biotinylated oligonucleotides.

AB - A series of non-nucleoslde-based 2,4-dlnltrophenyl (DNP) phosphoramidltes have been prepared and used in the multiple labelling of ollgonucleotldes during solid-phase synthesis. The length of spacer arm between the DNP label and the ollgonucleotlde phosphate backbone, and the number of attached DNP groups have both been varied In order to determine the optimum conditions for antl-DNP antibody binding. Detection using enzyme-linked colorimetrlc techniques showed sensitivity equivalent to that obtainable using biotinylated oligonucleotides.

UR - https://www.scopus.com/pages/publications/0027232499

U2 - 10.1093/nar/21.8.1705

DO - 10.1093/nar/21.8.1705

M3 - Article

SN - 0305-1048

VL - 21

SP - 1705

EP - 1712

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 8

ER -

Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups

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