Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups

J Grzybowski, DW Will, Richard Edward Randall, CA Smith, T Brown

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A series of non-nucleoslde-based 2,4-dlnltrophenyl (DNP) phosphoramidltes have been prepared and used in the multiple labelling of ollgonucleotldes during solid-phase synthesis. The length of spacer arm between the DNP label and the ollgonucleotlde phosphate backbone, and the number of attached DNP groups have both been varied In order to determine the optimum conditions for antl-DNP antibody binding. Detection using enzyme-linked colorimetrlc techniques showed sensitivity equivalent to that obtainable using biotinylated oligonucleotides.
Original languageEnglish
Pages (from-to)1705-1712
JournalNucleic Acids Research
Volume21
Issue number8
DOIs
Publication statusPublished - 25 Apr 1993

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