TY - JOUR
T1 - Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups
AU - Grzybowski, J
AU - Will, DW
AU - Randall, Richard Edward
AU - Smith, CA
AU - Brown, T
PY - 1993/4/25
Y1 - 1993/4/25
N2 - A series of non-nucleoslde-based 2,4-dlnltrophenyl (DNP) phosphoramidltes have been prepared and used in the multiple labelling of ollgonucleotldes during solid-phase synthesis. The length of spacer arm between the DNP label and the ollgonucleotlde phosphate backbone, and the number of attached DNP groups have both been varied In order to determine the optimum conditions for antl-DNP antibody binding. Detection using enzyme-linked colorimetrlc techniques showed sensitivity equivalent to that obtainable using biotinylated oligonucleotides.
AB - A series of non-nucleoslde-based 2,4-dlnltrophenyl (DNP) phosphoramidltes have been prepared and used in the multiple labelling of ollgonucleotldes during solid-phase synthesis. The length of spacer arm between the DNP label and the ollgonucleotlde phosphate backbone, and the number of attached DNP groups have both been varied In order to determine the optimum conditions for antl-DNP antibody binding. Detection using enzyme-linked colorimetrlc techniques showed sensitivity equivalent to that obtainable using biotinylated oligonucleotides.
UR - http://www.scopus.com/inward/record.url?scp=0027232499&partnerID=8YFLogxK
U2 - 10.1093/nar/21.8.1705
DO - 10.1093/nar/21.8.1705
M3 - Article
SN - 0305-1048
VL - 21
SP - 1705
EP - 1712
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 8
ER -