SUMO modification of the Ets-related transcription factor ERM inhibits its transcriptional activity

C Degerny, D Monte, C Beaudoin, E Jaffray, L Portois, R T Hay, Y de Launoit, J L Baert

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

A variety of transcription factors are post- translationally modified by SUMO, a 97- residue ubiquitin- like protein bound covalently to the targeted lysine. Here we describe SUMO modification of the Ets family member ERM at positions 89, 263, 293, and 350. To investigate how SUMO modification affects the function of ERM, Ets- responsive intercellular adhesion molecule 1 ( ICAM- 1) and E74 reporter plasmids were employed to demonstrate that SUMO modification causes inhibition of ERM- dependent transcription without affecting the subcellular localization, stability, or DNA- binding capacity of the protein. When the adenoviral protein Gam1 or the SUMO protease SENP1 was used to inhibit the SUMO modification pathway, ERM- dependent transcription was de- repressed. These results demonstrate that ERM is subject to SUMO modification and that this post- translational modification causes inhibition of transcription- enhancing activity.

Original languageEnglish
Pages (from-to)24330-24338
Number of pages9
JournalJournal of Biological Chemistry
Volume280
DOIs
Publication statusPublished - 1 Jul 2005

Keywords

  • CONJUGATING ENZYME UBC9
  • ANDROGEN RECEPTOR
  • IN-VIVO
  • HISTONE DEACETYLASE-1
  • COVALENT ATTACHMENT
  • FACTOR ER81
  • PEA3 GROUP
  • C-JUN
  • UBIQUITIN
  • NUCLEAR

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