Substrate specificity in enzymatic fluorination.  The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates

SL Cobb, H Deng, AR McEwan, RP McGlinchey, James Henderson Naismith, David O'Hagan, DA Robinson

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C - F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C - F bond formation.

Original languageEnglish
Pages (from-to)1458-1460
Number of pages3
JournalOrganic & Biomolecular Chemistry
Volume4
Issue number8
DOIs
Publication statusPublished - Apr 2006

Keywords

  • BOND FORMATION
  • CONVENIENT PREPARATION
  • BIOSYNTHESIS
  • REFINEMENT

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