Subcellular localization and interactions among TGB proteins of cowpea mild mottle virus

S L Carvalho; J Tilsner; A R Figueira; C M Carvalho

doi:10.1007/s00705-022-05576-7

Subcellular localization and interactions among TGB proteins of cowpea mild mottle virus

S L Carvalho, J Tilsner, A R Figueira, C M Carvalho^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Cowpea mild mottle virus (CPMMV) is a flexuous filamentous virus that belongs to the genus Carlavirus (family Betaflexiviridae). The CPMMV genome contains six open reading frames (ORFs), among which the triple gene block (TGB), encoded by ORFs 2 to 4, has been reported to encode movement proteins for different viruses. The subcellular localization of the TGB proteins of CPMMV isolate CPMMV:BR:MG:09:2 was analysed by transient expression of each protein fused to a fluorophore. Overall, the accumulation pattern and interactions among CPMMV TGB proteins (TGBp) were similar to those of their counterparts from the potex-like group. Considering these similarities, we evaluated the potential interactions between the TGB proteins of CPMMV and of potato virus X, which could complement cell-to-cell movement. The TGBp2 and TGBp3 of PVX had an effect on CPMMV TGBp1, directing it to the plasmodesmata, but the reverse was not true.

Original language	English
Pages (from-to)	2555-2566
Number of pages	12
Journal	Archives of Virology
Volume	167
Early online date	21 Oct 2022
DOIs	https://doi.org/10.1007/s00705-022-05576-7
Publication status	Published - 1 Dec 2022

Keywords

Cowpea mild mottle virus
Triple gene block
Subcellular localization

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10.1007/s00705-022-05576-7

Push on through to the other side: Push on through to the other side- molecular basis of viral cell-to-cell movement in plants
Tilsner, J. (PI)
BBSRC
1/03/15 → 31/01/19
Project: Standard

Cite this

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title = "Subcellular localization and interactions among TGB proteins of cowpea mild mottle virus",

abstract = "Cowpea mild mottle virus (CPMMV) is a flexuous filamentous virus that belongs to the genus Carlavirus (family Betaflexiviridae). The CPMMV genome contains six open reading frames (ORFs), among which the triple gene block (TGB), encoded by ORFs 2 to 4, has been reported to encode movement proteins for different viruses. The subcellular localization of the TGB proteins of CPMMV isolate CPMMV:BR:MG:09:2 was analysed by transient expression of each protein fused to a fluorophore. Overall, the accumulation pattern and interactions among CPMMV TGB proteins (TGBp) were similar to those of their counterparts from the potex-like group. Considering these similarities, we evaluated the potential interactions between the TGB proteins of CPMMV and of potato virus X, which could complement cell-to-cell movement. The TGBp2 and TGBp3 of PVX had an effect on CPMMV TGBp1, directing it to the plasmodesmata, but the reverse was not true.",

keywords = "Cowpea mild mottle virus, Triple gene block, Subcellular localization",

author = "Carvalho, {S L} and J Tilsner and Figueira, {A R} and Carvalho, {C M}",

note = "Funding: This work was funded by Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de Minas Gerais (FAPEMIG) Processo: APQ-01604-14, Brazil. S.L.C. was supported by a scholarship from the Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior, Brasil. Parts of this work were funded by the U.K. Biotechnology and Biomedical Sciences Research Council (BBSRC) grant BB/M007200/1 to J.T. Work in the J.T. laboratory is supported by the Scottish Government{\textquoteright}s Rural and Environment Science and Analytical Services Division (RESAS).",

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doi = "10.1007/s00705-022-05576-7",

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AU - Carvalho, S L

AU - Tilsner, J

AU - Figueira, A R

AU - Carvalho, C M

N1 - Funding: This work was funded by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Processo: APQ-01604-14, Brazil. S.L.C. was supported by a scholarship from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil. Parts of this work were funded by the U.K. Biotechnology and Biomedical Sciences Research Council (BBSRC) grant BB/M007200/1 to J.T. Work in the J.T. laboratory is supported by the Scottish Government’s Rural and Environment Science and Analytical Services Division (RESAS).

PY - 2022/12/1

Y1 - 2022/12/1

N2 - Cowpea mild mottle virus (CPMMV) is a flexuous filamentous virus that belongs to the genus Carlavirus (family Betaflexiviridae). The CPMMV genome contains six open reading frames (ORFs), among which the triple gene block (TGB), encoded by ORFs 2 to 4, has been reported to encode movement proteins for different viruses. The subcellular localization of the TGB proteins of CPMMV isolate CPMMV:BR:MG:09:2 was analysed by transient expression of each protein fused to a fluorophore. Overall, the accumulation pattern and interactions among CPMMV TGB proteins (TGBp) were similar to those of their counterparts from the potex-like group. Considering these similarities, we evaluated the potential interactions between the TGB proteins of CPMMV and of potato virus X, which could complement cell-to-cell movement. The TGBp2 and TGBp3 of PVX had an effect on CPMMV TGBp1, directing it to the plasmodesmata, but the reverse was not true.

AB - Cowpea mild mottle virus (CPMMV) is a flexuous filamentous virus that belongs to the genus Carlavirus (family Betaflexiviridae). The CPMMV genome contains six open reading frames (ORFs), among which the triple gene block (TGB), encoded by ORFs 2 to 4, has been reported to encode movement proteins for different viruses. The subcellular localization of the TGB proteins of CPMMV isolate CPMMV:BR:MG:09:2 was analysed by transient expression of each protein fused to a fluorophore. Overall, the accumulation pattern and interactions among CPMMV TGB proteins (TGBp) were similar to those of their counterparts from the potex-like group. Considering these similarities, we evaluated the potential interactions between the TGB proteins of CPMMV and of potato virus X, which could complement cell-to-cell movement. The TGBp2 and TGBp3 of PVX had an effect on CPMMV TGBp1, directing it to the plasmodesmata, but the reverse was not true.

KW - Cowpea mild mottle virus

KW - Triple gene block

KW - Subcellular localization

U2 - 10.1007/s00705-022-05576-7

DO - 10.1007/s00705-022-05576-7

M3 - Article

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SN - 0304-8608

VL - 167

SP - 2555

EP - 2566

JO - Archives of Virology

JF - Archives of Virology

ER -

Subcellular localization and interactions among TGB proteins of cowpea mild mottle virus

Abstract

Keywords

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Push on through to the other side: Push on through to the other side- molecular basis of viral cell-to-cell movement in plants

Cite this