Structure of the CRISPR Interference complex CSM reveals key similarities with Cascade

Christophe Rouillon; Min Zhou; Jing Zhang; Politis Argyris; Victoria Beilsten-Edmands; Guiseppe Cannone; Shirley Graham; Carol Robinson; Laura Spagnolo; Malcolm F White

doi:10.1016/j.molcel.2013.08.020

Structure of the CRISPR Interference complex CSM reveals key similarities with Cascade

Christophe Rouillon, Min Zhou, Jing Zhang, Politis Argyris, Victoria Beilsten-Edmands, Guiseppe Cannone, Shirley Graham, Carol Robinson, Laura Spagnolo, Malcolm F White

Research output: Contribution to journal › Article › peer-review

Abstract

The Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system is an adaptive immune system in prokaryotes. Interference complexes encoded by CRISPR-associated (cas) genes utilize small RNAs for homology-directed detection and subsequent degradation of invading genetic elements, and they have been classified into three main types (I–III). Type III complexes share the Cas10 subunit but are subclassifed as type IIIA (CSM) and type IIIB (CMR), depending on their specificity for DNA or RNA targets, respectively. The role of CSM in limiting the spread of conjugative plasmids in Staphylococcus epidermidis was first described in 2008. Here, we report a detailed investigation of the composition and structure of the CSM complex from the archaeon Sulfolobus solfataricus, using a combination of electron microscopy, mass spectrometry, and deep sequencing. This reveals a three-dimensional model for the CSM complex that includes a helical component strikingly reminiscent of the backbone structure of the type I (Cascade) family.

Original language	English
Pages (from-to)	124-134
Number of pages	11
Journal	Molecular Cell
Volume	52
Issue number	1
Early online date	10 Oct 2013
DOIs	https://doi.org/10.1016/j.molcel.2013.08.020
Publication status	Published - 10 Oct 2013

Keywords

Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system
Immune system
Prokaryotes
CSM complex
Sulfolobus solfataricus
Cascade complex
Methylation
Acetylation
Phosphorylation

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10.1016/j.molcel.2013.08.020

MolecularCell2013
Copyright © 2013 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Final published version, 1.9 MB

The CMR complex for prokaryotic RNA: The CMR complex for prokaryotic RNA silencing
White, M. (PI)
BBSRC
1/09/12 → 31/10/15
Project: Standard
Elucidating the molecular architecture: Elucidating the molecular architecture of the Archaeal CMR complex, a key player in yhr unicellular immune response.
White, M. (PI)
BBSRC
1/08/12 → 31/07/15
Project: Standard

Cite this

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title = "Structure of the CRISPR Interference complex CSM reveals key similarities with Cascade",

abstract = "The Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system is an adaptive immune system in prokaryotes. Interference complexes encoded by CRISPR-associated (cas) genes utilize small RNAs for homology-directed detection and subsequent degradation of invading genetic elements, and they have been classified into three main types (I–III). Type III complexes share the Cas10 subunit but are subclassifed as type IIIA (CSM) and type IIIB (CMR), depending on their specificity for DNA or RNA targets, respectively. The role of CSM in limiting the spread of conjugative plasmids in Staphylococcus epidermidis was first described in 2008. Here, we report a detailed investigation of the composition and structure of the CSM complex from the archaeon Sulfolobus solfataricus, using a combination of electron microscopy, mass spectrometry, and deep sequencing. This reveals a three-dimensional model for the CSM complex that includes a helical component strikingly reminiscent of the backbone structure of the type I (Cascade) family.",

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AU - Cannone, Guiseppe

AU - Graham, Shirley

AU - Robinson, Carol

AU - Spagnolo, Laura

AU - White, Malcolm F

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AB - The Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system is an adaptive immune system in prokaryotes. Interference complexes encoded by CRISPR-associated (cas) genes utilize small RNAs for homology-directed detection and subsequent degradation of invading genetic elements, and they have been classified into three main types (I–III). Type III complexes share the Cas10 subunit but are subclassifed as type IIIA (CSM) and type IIIB (CMR), depending on their specificity for DNA or RNA targets, respectively. The role of CSM in limiting the spread of conjugative plasmids in Staphylococcus epidermidis was first described in 2008. Here, we report a detailed investigation of the composition and structure of the CSM complex from the archaeon Sulfolobus solfataricus, using a combination of electron microscopy, mass spectrometry, and deep sequencing. This reveals a three-dimensional model for the CSM complex that includes a helical component strikingly reminiscent of the backbone structure of the type I (Cascade) family.

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KW - Prokaryotes

KW - CSM complex

KW - Sulfolobus solfataricus

KW - Cascade complex

KW - Methylation

KW - Acetylation

KW - Phosphorylation

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DO - 10.1016/j.molcel.2013.08.020

M3 - Article

SN - 1097-2765

VL - 52

SP - 124

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JO - Molecular Cell

JF - Molecular Cell

IS - 1

ER -

Structure of the CRISPR Interference complex CSM reveals key similarities with Cascade

Abstract

Keywords

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The CMR complex for prokaryotic RNA: The CMR complex for prokaryotic RNA silencing

Elucidating the molecular architecture: Elucidating the molecular architecture of the Archaeal CMR complex, a key player in yhr unicellular immune response.

Cite this