Structure and mechanism of the CMR complex for CRISPR-Mediated antiviral immunity

Jing Zhang, Christophe Rouillon, Melina Kerou, Judith Reeks, Kim Brugger, Shirley Graham, Julia Reimann, Giuseppe Cannone, Huanting Liu, Sonja-Verena Albers, James H. Naismith, Laura Spagnolo, Malcolm F White

Research output: Contribution to journalArticlepeer-review

259 Citations (Scopus)
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Abstract

The prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading viruses and incorporated into ribonucleoprotein complexes with CRISPR-associated (CAS) proteins, to target and degrade viral DNA or RNA on subsequent infection. RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse "payload" of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endo-nucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5' sequence in the crRNA, but not on the presence of a proto-spacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets.

Original languageEnglish
Pages (from-to)303-313
Number of pages11
JournalMolecular Cell
Volume45
Issue number3
DOIs
Publication statusPublished - 10 Feb 2012

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