Structural snapshots of the reaction coordinate for O-GlcNAc transferase

Michael B. Lazarus, Jiaoyang Jiang, Tracey M. Gloster, Wesley F. Zandberg, Garrett E. Whitworth, David J. Vocadlo, Suzanne Walker

Research output: Contribution to journalArticlepeer-review

94 Citations (Scopus)

Abstract

Visualization of the reaction coordinate undertaken by glycosyltransferases has remained elusive but is critical for understanding this important class of enzyme. Using substrates and substrate mimics, we describe structural snapshots of all species along the kinetic pathway for human O-linked beta-N-acetylglucosamine transferase (O-GlcNAc transferase), an intracellular enzyme that catalyzes installation of a dynamic post-translational modification. The structures reveal key features of the mechanism and show that substrate participation is important during catalysis.

Original languageEnglish
Pages (from-to)966-968
Number of pages3
JournalNature Chemical Biology
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 2012

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