Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Yoshihiko Arita; Maria Leilani Torres-Mapa; Woei Ming Lee; Tomas Cizmar; Paul A Campbell; Frank J Gunn-Moore; Kishan Dholakia

doi:10.1063/1.3554415

Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Yoshihiko Arita, Maria Leilani Torres-Mapa, Woei Ming Lee, Tomas Cizmar, Paul A Campbell, Frank J Gunn-Moore, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

Original language	English
Article number	093702
Number of pages	3
Journal	Applied Physics Letters
Volume	98
Issue number	9
DOIs	https://doi.org/10.1063/1.3554415
Publication status	Published - Mar 2011

Keywords

Nanoparticles
Cell membranes
Cavitation
Cell membrane transport
Monolayers

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10.1063/1.3554415

1.3554415
Copyright 2011, American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Applied Physics Letters, Vol 98, Issue 9, and may be found at: http://scitation.aip.org/content/aip/journal/apl/98/9/10.1063/1.3554415
Final published version, 692 KB

EP/G029733/1 - Plasmon enhanced manipula: Plasmon enhanced manipulation and sorting of nanoparticles
Dholakia, K. (PI)
EPSRC
1/06/09 → 30/11/12
Project: Standard

Cite this

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title = "Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles",

abstract = "We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.",

keywords = "Nanoparticles, Cell membranes, Cavitation, Cell membrane transport, Monolayers",

author = "Yoshihiko Arita and Torres-Mapa, {Maria Leilani} and Lee, {Woei Ming} and Tomas Cizmar and Campbell, {Paul A} and Gunn-Moore, {Frank J} and Kishan Dholakia",

year = "2011",

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doi = "10.1063/1.3554415",

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AU - Arita, Yoshihiko

AU - Torres-Mapa, Maria Leilani

AU - Lee, Woei Ming

AU - Cizmar, Tomas

AU - Campbell, Paul A

AU - Gunn-Moore, Frank J

AU - Dholakia, Kishan

PY - 2011/3

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N2 - We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

AB - We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

KW - Nanoparticles

KW - Cell membranes

KW - Cavitation

KW - Cell membrane transport

KW - Monolayers

U2 - 10.1063/1.3554415

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M3 - Article

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VL - 98

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 9

M1 - 093702

ER -

Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

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Keywords

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EP/G029733/1 - Plasmon enhanced manipula: Plasmon enhanced manipulation and sorting of nanoparticles

Cite this