Projects per year
We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.
- Cell membranes
- Cell membrane transport
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- 1 Finished
EP/G029733/1 - Plasmon enhanced manipula: Plasmon enhanced manipulation and sorting of nanoparticles
1/06/09 → 30/11/12