Snapshot hyperspectral imaging of intracellular lasers

Soraya Caixeiro *, Philip Wijesinghe, Kishan Dholakia, Malte Christian Gather*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Intracellular lasers are emerging as powerful biosensors for multiplexed tracking and precision sensing of cells and their microenvironment. This sensing capacity is enabled by quantifying their narrow-linewidth emission spectra, which is presently challenging to do at high speeds. In this work, we demonstrate rapid snapshot hyperspectral imaging of intracellular lasers. Using integral field mapping with a microlens array and a diffraction grating, we obtain images of the spatial and spectral intensity distribution from a single camera acquisition. We demonstrate widefield hyperspectral imaging over a 3 × 3 mm2 field of view and volumetric imaging over 250 × 250 × 800 µm3 (XYZ) volumes with a lateral (XY) resolution of 5 µm, axial (Z) resolution of 10 µm, and a spectral resolution of less than 0.8 nm. We evaluate the performance and outline the challenges and strengths of snapshot methods in the context of characterizing the emission from intracellular lasers. This method offers new opportunities for a diverse range of applications, including high-throughput and long-term biosensing with intracellular lasers.

Original languageEnglish
Number of pages16
JournalOptics Express
Volume31
Issue number20
DOIs
Publication statusPublished - 20 Sept 2023

Keywords

  • Whispering gallery mode lasers
  • Biolaser
  • Intracellular lasing
  • Laser particles
  • Integral field mapping

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