Size Selective Protein Adsorption on Thiol-functionalised SBA-15 Mesoporous Molecular Sieve

HH Yiu; Catherine Helen Botting; Nigel Peter Botting; Paul Anthony Wright

doi:10.1039/b104729a

Size Selective Protein Adsorption on Thiol-functionalised SBA-15 Mesoporous Molecular Sieve

HH Yiu, Catherine Helen Botting, Nigel Peter Botting, Paul Anthony Wright

Research output: Other contribution

Abstract

The mesoporous silica SBA-15, functionalised with propylthiol groups during synthesis and rendered porous (mean pore diameter 51 Angstrom) by extraction of surfactant template molecules, shows strong and size selective adsorption of proteins, selectively excluding those with molecular weights of ca. 40000 u and above. A model for the adsorption process is proposed, in which reversible physisorption is followed by irreversible chemisorption. Adsorption of proteins on an unfunctionalised SBA-15 from which the template has been removed by calcination, (mean pore diameter 56 Angstrom) shows shape selective and reversible adsorption of proteins with molecular weights of ca. 43000 u and below.

Original language	English
Volume	3
DOIs	https://doi.org/10.1039/b104729a
Publication status	Published - 2001

Keywords

IMMOBILIZED ENZYMES
CRYSTAL-STRUCTURE
SILICA
COPOLYMER
TRIBLOCK
RESOLUTION
OVALBUMIN
CHEMISTRY

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10.1039/b104729a

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@misc{2f5ad31e4d7049179b911f6e0de6f4c8,

title = "Size Selective Protein Adsorption on Thiol-functionalised SBA-15 Mesoporous Molecular Sieve",

abstract = "The mesoporous silica SBA-15, functionalised with propylthiol groups during synthesis and rendered porous (mean pore diameter 51 Angstrom) by extraction of surfactant template molecules, shows strong and size selective adsorption of proteins, selectively excluding those with molecular weights of ca. 40000 u and above. A model for the adsorption process is proposed, in which reversible physisorption is followed by irreversible chemisorption. Adsorption of proteins on an unfunctionalised SBA-15 from which the template has been removed by calcination, (mean pore diameter 56 Angstrom) shows shape selective and reversible adsorption of proteins with molecular weights of ca. 43000 u and below.",

keywords = "IMMOBILIZED ENZYMES, CRYSTAL-STRUCTURE, SILICA, COPOLYMER, TRIBLOCK, RESOLUTION, OVALBUMIN, CHEMISTRY",

author = "HH Yiu and Botting, {Catherine Helen} and Botting, {Nigel Peter} and Wright, {Paul Anthony}",

note = "Phys Chem Chem Phys",

year = "2001",

doi = "10.1039/b104729a",

language = "English",

volume = "3",

type = "Other",

}

TY - GEN

T1 - Size Selective Protein Adsorption on Thiol-functionalised SBA-15 Mesoporous Molecular Sieve

AU - Yiu, HH

AU - Botting, Catherine Helen

AU - Botting, Nigel Peter

AU - Wright, Paul Anthony

N1 - Phys Chem Chem Phys

PY - 2001

Y1 - 2001

N2 - The mesoporous silica SBA-15, functionalised with propylthiol groups during synthesis and rendered porous (mean pore diameter 51 Angstrom) by extraction of surfactant template molecules, shows strong and size selective adsorption of proteins, selectively excluding those with molecular weights of ca. 40000 u and above. A model for the adsorption process is proposed, in which reversible physisorption is followed by irreversible chemisorption. Adsorption of proteins on an unfunctionalised SBA-15 from which the template has been removed by calcination, (mean pore diameter 56 Angstrom) shows shape selective and reversible adsorption of proteins with molecular weights of ca. 43000 u and below.

AB - The mesoporous silica SBA-15, functionalised with propylthiol groups during synthesis and rendered porous (mean pore diameter 51 Angstrom) by extraction of surfactant template molecules, shows strong and size selective adsorption of proteins, selectively excluding those with molecular weights of ca. 40000 u and above. A model for the adsorption process is proposed, in which reversible physisorption is followed by irreversible chemisorption. Adsorption of proteins on an unfunctionalised SBA-15 from which the template has been removed by calcination, (mean pore diameter 56 Angstrom) shows shape selective and reversible adsorption of proteins with molecular weights of ca. 43000 u and below.

KW - IMMOBILIZED ENZYMES

KW - CRYSTAL-STRUCTURE

KW - SILICA

KW - COPOLYMER

KW - TRIBLOCK

KW - RESOLUTION

KW - OVALBUMIN

KW - CHEMISTRY

UR - http://www.scopus.com/inward/record.url?scp=0034827978&partnerID=8YFLogxK

UR - http://www.rsc.org/delivery/_ArticleLinking/DisplayArticleForFree.cfm?doi=b104729a&JournalCode=CP

U2 - 10.1039/b104729a

DO - 10.1039/b104729a

M3 - Other contribution

VL - 3

ER -

Size Selective Protein Adsorption on Thiol-functionalised SBA-15 Mesoporous Molecular Sieve

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Keywords

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