Simple blood-spot sampling with nested polymerase chain reaction detection for epidemiology studies on Brugia malayi

J  Cox-Singh; A S  Pomrehn; H A  Rahman; R  Zakaria; A O  Miller; B  Singh; Janet Cox Singh

Simple blood-spot sampling with nested polymerase chain reaction detection for epidemiology studies on Brugia malayi

J Cox-Singh, A S Pomrehn, H A Rahman, R Zakaria, A O Miller, B Singh, Janet Cox Singh

Research output: Contribution to journal › Article › peer-review

Abstract

In the absence of a suitable Brugia malayi antigen detection assay, PCR remains one of the more sensitive alternatives to Giemsa-stained thick blood films for B. malayi detection. The need for refrigerated storage and transportation of blood has limited the use of PCR for large-scale epidemiology studies in remote endemic areas. Here we report simple finger-prick blood-spot collection, a one-tube DNA template extraction method and the development of a B. malayi-specific nested PCR assay. The assay was tested on 145 field samples and was positive for all 30 microscopy-positive samples and for an additional 13 samples which were microscopy-negative. (C) 1999 Australian Society for Parasitology. Published by Elsevier Science Ltd. All rights reserved.

Original language	English
Pages (from-to)	717-721
Number of pages	5
Journal	International Journal for Parasitology
Volume	29
Issue number	5
Publication status	Published - May 1999

Cite this

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title = "Simple blood-spot sampling with nested polymerase chain reaction detection for epidemiology studies on Brugia malayi",

abstract = "In the absence of a suitable Brugia malayi antigen detection assay, PCR remains one of the more sensitive alternatives to Giemsa-stained thick blood films for B. malayi detection. The need for refrigerated storage and transportation of blood has limited the use of PCR for large-scale epidemiology studies in remote endemic areas. Here we report simple finger-prick blood-spot collection, a one-tube DNA template extraction method and the development of a B. malayi-specific nested PCR assay. The assay was tested on 145 field samples and was positive for all 30 microscopy-positive samples and for an additional 13 samples which were microscopy-negative. (C) 1999 Australian Society for Parasitology. Published by Elsevier Science Ltd. All rights reserved.",

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AU - Cox-Singh, J

AU - Pomrehn, A S

AU - Rahman, H A

AU - Zakaria, R

AU - Miller, A O

AU - Singh, B

AU - Cox Singh, Janet

PY - 1999/5

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N2 - In the absence of a suitable Brugia malayi antigen detection assay, PCR remains one of the more sensitive alternatives to Giemsa-stained thick blood films for B. malayi detection. The need for refrigerated storage and transportation of blood has limited the use of PCR for large-scale epidemiology studies in remote endemic areas. Here we report simple finger-prick blood-spot collection, a one-tube DNA template extraction method and the development of a B. malayi-specific nested PCR assay. The assay was tested on 145 field samples and was positive for all 30 microscopy-positive samples and for an additional 13 samples which were microscopy-negative. (C) 1999 Australian Society for Parasitology. Published by Elsevier Science Ltd. All rights reserved.

AB - In the absence of a suitable Brugia malayi antigen detection assay, PCR remains one of the more sensitive alternatives to Giemsa-stained thick blood films for B. malayi detection. The need for refrigerated storage and transportation of blood has limited the use of PCR for large-scale epidemiology studies in remote endemic areas. Here we report simple finger-prick blood-spot collection, a one-tube DNA template extraction method and the development of a B. malayi-specific nested PCR assay. The assay was tested on 145 field samples and was positive for all 30 microscopy-positive samples and for an additional 13 samples which were microscopy-negative. (C) 1999 Australian Society for Parasitology. Published by Elsevier Science Ltd. All rights reserved.

M3 - Article

SN - 0020-7519

VL - 29

SP - 717

EP - 721

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 5

ER -

Simple blood-spot sampling with nested polymerase chain reaction detection for epidemiology studies on Brugia malayi

Abstract

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