Simple blood-spot sampling with nested polymerase chain reaction detection for epidemiology studies on Brugia malayi

J Cox-Singh, A S Pomrehn, H A Rahman, R Zakaria, A O Miller, B Singh, Janet Cox Singh

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

In the absence of a suitable Brugia malayi antigen detection assay, PCR remains one of the more sensitive alternatives to Giemsa-stained thick blood films for B. malayi detection. The need for refrigerated storage and transportation of blood has limited the use of PCR for large-scale epidemiology studies in remote endemic areas. Here we report simple finger-prick blood-spot collection, a one-tube DNA template extraction method and the development of a B. malayi-specific nested PCR assay. The assay was tested on 145 field samples and was positive for all 30 microscopy-positive samples and for an additional 13 samples which were microscopy-negative. (C) 1999 Australian Society for Parasitology. Published by Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)717-721
Number of pages5
JournalInternational Journal for Parasitology
Volume29
Issue number5
Publication statusPublished - May 1999

Fingerprint

Dive into the research topics of 'Simple blood-spot sampling with nested polymerase chain reaction detection for epidemiology studies on Brugia malayi'. Together they form a unique fingerprint.

Cite this