Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

Chetna Taneja; Jerin Geogy George; Stella Corsetti; Philip Wijesinghe; Graham D. Bruce; Maarten F. Zwart; Shanti Bhattacharya; Kishan Dholakia

doi:10.1364/BOE.538253

Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

Chetna Taneja, Jerin Geogy George, Stella Corsetti, Philip Wijesinghe, Graham D. Bruce, Maarten F. Zwart, Shanti Bhattacharya, Kishan Dholakia^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The Bessel beam (BB) has found widespread adoption in various forms of light-sheet microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses challenges due to the detrimental effect of the sidelobes. Here, we mitigate this issue by using a computer-generated phase element for generating a sidelobe suppressed Bessel beam (SSBB). We then progress to perform a comparison of biological imaging using SSBB to standard BB in a light-sheet geometry. The SSBB peak intensity is more than an order of magnitude higher than the first sidelobe. In contrast to a standard BB light-sheet, an SSBB does not need deconvolution. The SSBB propagates to depths exceeding 400 μm in phantom samples maintaining a transverse size of 5 μm. Finally, we demonstrate the advantage of using an SSBB light-sheet for biological applications by imaging fixed early-stage zebrafish larvae. In comparison to the standard BB, we observe a two-fold increase in contrast-to-noise ratio (CNR) when imaging the labelled cellular eye structures and the notochords. Our results provide an effective approach to generating and using SSBB light-sheets to enhance contrast for one-photon light-sheet microscopy.

Original language	English
Pages (from-to)	6183-6197
Number of pages	15
Journal	Biomedical Optics Express
Volume	15
Issue number	11
Early online date	4 Oct 2024
DOIs	https://doi.org/10.1364/BOE.538253
Publication status	Published - 1 Nov 2024

Keywords

Biomedical imaging
Imaging systems
Optical imaging
Phase modulation
Spatial light modulators
Three dimensional reconstruction

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Copyright © 2024 the Authors. Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.
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title = "Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy",

abstract = "The Bessel beam (BB) has found widespread adoption in various forms of light-sheet microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses challenges due to the detrimental effect of the sidelobes. Here, we mitigate this issue by using a computer-generated phase element for generating a sidelobe suppressed Bessel beam (SSBB). We then progress to perform a comparison of biological imaging using SSBB to standard BB in a light-sheet geometry. The SSBB peak intensity is more than an order of magnitude higher than the first sidelobe. In contrast to a standard BB light-sheet, an SSBB does not need deconvolution. The SSBB propagates to depths exceeding 400 μm in phantom samples maintaining a transverse size of 5 μm. Finally, we demonstrate the advantage of using an SSBB light-sheet for biological applications by imaging fixed early-stage zebrafish larvae. In comparison to the standard BB, we observe a two-fold increase in contrast-to-noise ratio (CNR) when imaging the labelled cellular eye structures and the notochords. Our results provide an effective approach to generating and using SSBB light-sheets to enhance contrast for one-photon light-sheet microscopy.",

keywords = "Biomedical imaging, Imaging systems, Optical imaging, Phase modulation, Spatial light modulators, Three dimensional reconstruction",

author = "Chetna Taneja and George, \{Jerin Geogy\} and Stella Corsetti and Philip Wijesinghe and Bruce, \{Graham D.\} and Zwart, \{Maarten F.\} and Shanti Bhattacharya and Kishan Dholakia",

note = "Funding: Engineering and Physical Sciences Research Council (EP/R004854/1); HORIZON EUROPE Innovative Europe (EC-GA863203); Australian Research Council (FL210100099); Scheme for Promotion of Academic and Research Collaboration (SPARC/2018-2019/P796/SL); Biotechnology and Biological Sciences Research Council (BB/T006560/1); Royal Commission for the Exhibition of 1851 (Research fellowship).",

year = "2024",

month = nov,

day = "1",

doi = "10.1364/BOE.538253",

language = "English",

volume = "15",

pages = "6183--6197",

journal = "Biomedical Optics Express",

issn = "2156-7085",

publisher = "Optica Publishing Group (formerly OSA)",

number = "11",

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TY - JOUR

T1 - Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

AU - Taneja, Chetna

AU - George, Jerin Geogy

AU - Corsetti, Stella

AU - Wijesinghe, Philip

AU - Bruce, Graham D.

AU - Zwart, Maarten F.

AU - Bhattacharya, Shanti

AU - Dholakia, Kishan

N1 - Funding: Engineering and Physical Sciences Research Council (EP/R004854/1); HORIZON EUROPE Innovative Europe (EC-GA863203); Australian Research Council (FL210100099); Scheme for Promotion of Academic and Research Collaboration (SPARC/2018-2019/P796/SL); Biotechnology and Biological Sciences Research Council (BB/T006560/1); Royal Commission for the Exhibition of 1851 (Research fellowship).

PY - 2024/11/1

Y1 - 2024/11/1

N2 - The Bessel beam (BB) has found widespread adoption in various forms of light-sheet microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses challenges due to the detrimental effect of the sidelobes. Here, we mitigate this issue by using a computer-generated phase element for generating a sidelobe suppressed Bessel beam (SSBB). We then progress to perform a comparison of biological imaging using SSBB to standard BB in a light-sheet geometry. The SSBB peak intensity is more than an order of magnitude higher than the first sidelobe. In contrast to a standard BB light-sheet, an SSBB does not need deconvolution. The SSBB propagates to depths exceeding 400 μm in phantom samples maintaining a transverse size of 5 μm. Finally, we demonstrate the advantage of using an SSBB light-sheet for biological applications by imaging fixed early-stage zebrafish larvae. In comparison to the standard BB, we observe a two-fold increase in contrast-to-noise ratio (CNR) when imaging the labelled cellular eye structures and the notochords. Our results provide an effective approach to generating and using SSBB light-sheets to enhance contrast for one-photon light-sheet microscopy.

AB - The Bessel beam (BB) has found widespread adoption in various forms of light-sheet microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses challenges due to the detrimental effect of the sidelobes. Here, we mitigate this issue by using a computer-generated phase element for generating a sidelobe suppressed Bessel beam (SSBB). We then progress to perform a comparison of biological imaging using SSBB to standard BB in a light-sheet geometry. The SSBB peak intensity is more than an order of magnitude higher than the first sidelobe. In contrast to a standard BB light-sheet, an SSBB does not need deconvolution. The SSBB propagates to depths exceeding 400 μm in phantom samples maintaining a transverse size of 5 μm. Finally, we demonstrate the advantage of using an SSBB light-sheet for biological applications by imaging fixed early-stage zebrafish larvae. In comparison to the standard BB, we observe a two-fold increase in contrast-to-noise ratio (CNR) when imaging the labelled cellular eye structures and the notochords. Our results provide an effective approach to generating and using SSBB light-sheets to enhance contrast for one-photon light-sheet microscopy.

KW - Biomedical imaging

KW - Imaging systems

KW - Optical imaging

KW - Phase modulation

KW - Spatial light modulators

KW - Three dimensional reconstruction

UR - https://www.scopus.com/pages/publications/85209144845

U2 - 10.1364/BOE.538253

DO - 10.1364/BOE.538253

M3 - Article

SN - 2156-7085

VL - 15

SP - 6183

EP - 6197

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 11

ER -

Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

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