Projects per year
Abstract
The Bessel beam (BB) has found widespread adoption in various forms of light-sheet microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses challenges due to the detrimental effect of the sidelobes. Here, we mitigate this issue by using a computer-generated phase element for generating a sidelobe suppressed Bessel beam (SSBB). We then progress to perform a comparison of biological imaging using SSBB to standard BB in a light-sheet geometry. The SSBB peak intensity is more than an order of magnitude higher than the first sidelobe. In contrast to a standard BB light-sheet, an SSBB does not need deconvolution. The SSBB propagates to depths exceeding 400 μm in phantom samples maintaining a transverse size of 5 μm. Finally, we demonstrate the advantage of using an SSBB light-sheet for biological applications by imaging fixed early-stage zebrafish larvae. In comparison to the standard BB, we observe a two-fold increase in contrast-to-noise ratio (CNR) when imaging the labelled cellular eye structures and the notochords. Our results provide an effective approach to generating and using SSBB light-sheets to enhance contrast for one-photon light-sheet microscopy.
Original language | English |
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Pages (from-to) | 6183-6197 |
Number of pages | 15 |
Journal | Biomedical Optics Express |
Volume | 15 |
Issue number | 11 |
Early online date | 4 Oct 2024 |
DOIs | |
Publication status | Published - 1 Nov 2024 |
Keywords
- Biomedical imaging
- Imaging systems
- Optical imaging
- Phase modulation
- Spatial light modulators
- Three dimensional reconstruction
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Dive into the research topics of 'Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy'. Together they form a unique fingerprint.Projects
- 3 Finished
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Prosperity Partnership Additional: Prosperity Partnership Additional Allocation Offer
Dholakia, K. (PI)
14/12/23 → 31/03/24
Project: Standard
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H2020-FETOPEN-2018-2020. DynAMic: H2020-FETOPEN-2018-2020. DynAMic
Dholakia, K. (PI)
1/01/20 → 31/12/23
Project: Standard
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M Squared - USTAN Biophotonics Nexus: M Sqaured - St Andrews Biophotonics Nexus
Dholakia, K. (PI)
1/11/17 → 31/10/22
Project: Standard
Datasets
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Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy (dataset)
Taneja, C. (Creator), George, J. G. (Creator), Corsetti, S. (Creator), Wijesinghe, P. (Creator), Bruce, G. D. (Creator), Zwart, M. F. (Creator), Bhattacharya, S. (Creator) & Dholakia, K. (Creator), University of St Andrews, 3 Dec 2024
DOI: 10.17630/92a0783e-2fd3-43ca-bce7-ccfeb0bd5732
Dataset
File