Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

Chetna Taneja, Jerin Geogy George, Stella Corsetti, Philip Wijesinghe, Graham D. Bruce, Maarten F. Zwart, Shanti Bhattacharya, Kishan Dholakia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The Bessel beam (BB) has found widespread adoption in various forms of light-sheet microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses challenges due to the detrimental effect of the sidelobes. Here, we mitigate this issue by using a computer-generated phase element for generating a sidelobe suppressed Bessel beam (SSBB). We then progress to perform a comparison of biological imaging using SSBB to standard BB in a light-sheet geometry. The SSBB peak intensity is more than an order of magnitude higher than the first sidelobe. In contrast to a standard BB light-sheet, an SSBB does not need deconvolution. The SSBB propagates to depths exceeding 400 μm in phantom samples maintaining a transverse size of 5 μm. Finally, we demonstrate the advantage of using an SSBB light-sheet for biological applications by imaging fixed early-stage zebrafish larvae. In comparison to the standard BB, we observe a two-fold increase in contrast-to-noise ratio (CNR) when imaging the labelled cellular eye structures and the notochords. Our results provide an effective approach to generating and using SSBB light-sheets to enhance contrast for one-photon light-sheet microscopy.
Original languageEnglish
Pages (from-to)6183-6197
Number of pages15
JournalBiomedical Optics Express
Volume15
Issue number11
Early online date4 Oct 2024
DOIs
Publication statusPublished - 1 Nov 2024

Keywords

  • Biomedical imaging
  • Imaging systems
  • Optical imaging
  • Phase modulation
  • Spatial light modulators
  • Three dimensional reconstruction

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