Sialic acid recognition by Vibrio cholerae neuraminidase

I Moustafa, Helen Connaris, M Taylor, V Zaitsev, JC Wilson, MJ Kiefel, M von Itzstein, Garry Lindsay Taylor

Research output: Contribution to journalArticlepeer-review

111 Citations (Scopus)

Abstract

Vibrio cholerae neuraminidase (VCNA) plays a significant role in the pathogenesis of cholera by removing sialic acid from higher order gangliosides to unmask GM1, the receptor for cholera toxin. We previously showed that the structure of VCNA is composed of a central beta-propeller catalytic domain flanked by two lectin-like domains; however the nature of the carbohydrates recognized by these lectin domains has remained unknown. We present here structures of the enzyme in complex with two substrates, alpha-2,3-sialyllactose and alpha-2,6-sialyllactose. Both substrate complexes reveal the alpha-anomer of N-acetylneuraminic acid (Neu5Ac) bound to the N-terminal lectin domain, thereby revealing the role of this domain. The large number of interactions suggest a relatively high binding affinity for sialic acid, which was confirmed by calorimetry, which gave a K-d similar to 30 muM. Saturation transfer difference NMR using a non-hydrolyzable substrate, Neu5,9Ac(2)-2-S(alpha-2,6)-GlcNAcbeta1Me, was also used to map the ligand interactions at the VCNA lectin binding site. It is well known that VCNA can hydrolyze both alpha-2,3- and alpha-2,6-linked sialic acid substrates. In this study using alpha-2,3-sialyllactose co-crystallized with VCNA it was revealed that the inhibitor 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (Neu5Ac2en) was bound at the catalytic site. This observation supports the notion that VCNA can produce its own inhibitor and has been further confirmed by H-1 NMR analysis. The discovery of the sialic acid binding site in the N-lectin-like domain suggests that this might help target VCNA to sialic acid-rich environments, thereby enhancing the catalytic efficiency of the enzyme.

Original languageEnglish
Pages (from-to)40819-40826
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number39
DOIs
Publication statusPublished - 24 Sept 2004

Keywords

  • NUCLEAR-MAGNETIC-RESONANCE
  • CARBOHYDRATE-BINDING SITE
  • WHEAT-GERM-AGGLUTININ
  • CRUZI TRANS-SIALIDASE
  • CRYSTAL-STRUCTURE
  • INFLUENZA-VIRUS
  • HEMAGGLUTININ-NEURAMINIDASE
  • BACTERIAL SIALIDASES
  • RECEPTOR-BINDING
  • PERTUSSIS TOXIN

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