Sharpening emitter localization in front of a tuned mirror

Hannah S. Heil; Benjamin Schreiber; Ralph Götz; Monika Emmerling; Marie-Christine Dabauvalle; Georg Krohne; Sven Höfling; Martin Kamp; Markus Sauer; Katrin G. Heinze

doi:10.1038/s41377-018-0104-z

Sharpening emitter localization in front of a tuned mirror

Hannah S. Heil, Benjamin Schreiber, Ralph Götz, Monika Emmerling, Marie-Christine Dabauvalle, Georg Krohne, Sven Höfling, Martin Kamp, Markus Sauer, Katrin G. Heinze

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Abstract

Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio¹. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tunedmirror^2–4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor oftwo. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.

Original language	English
Article number	99
Number of pages	8
Journal	Light: Science & Applications
Volume	7
DOIs	https://doi.org/10.1038/s41377-018-0104-z
Publication status	Published - 5 Dec 2018

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title = "Sharpening emitter localization in front of a tuned mirror",

abstract = "Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tunedmirror2–4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor oftwo. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.",

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AU - Heil, Hannah S.

AU - Schreiber, Benjamin

AU - Götz, Ralph

AU - Emmerling, Monika

AU - Dabauvalle, Marie-Christine

AU - Krohne, Georg

AU - Höfling, Sven

AU - Kamp, Martin

AU - Sauer, Markus

AU - Heinze, Katrin G.

PY - 2018/12/5

Y1 - 2018/12/5

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AB - Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tunedmirror2–4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor oftwo. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.

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DO - 10.1038/s41377-018-0104-z

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SN - 2047-7538

VL - 7

JO - Light: Science & Applications

JF - Light: Science & Applications

M1 - 99

ER -

Sharpening emitter localization in front of a tuned mirror

Abstract

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