Sequence, circulating levels, and expression of C-type natriuretic peptide in a euryhaline elasmobranch, Carcharhinus leucas

WG Anderson, S Hyodo, T Tsukada, L Meischke, RD Pillans, JP Good, Y Takei, Gordon Cramb, CE Franklin, Neil Hazon

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The present study has examined expression and circulating levels of C-type natriuretic peptide (CNP) in the euryhaline bull shark, Carcharhinus leucas. Complementary DNA and deduced amino acid sequence for CNP in C leucas were determined by RACE methods. Homology of CNP amino acid sequence in C. leucas was high both for proCNP and for mature CNP when compared with previously identified elasmobranch CNPs. Mature CNP sequence in C. leucas was identical to that in Triakis seyllia and Seyliorhinus canicula. Levels of expression of CNP mRNA were significantly decreased in the atrium but did not change in either the brain or ventricle following acclimation to a SW environment. However, circulating levels of CNP significantly increased from 86.0 +/- 7.9 fmol ml(-1) in FW to 144.9 +/- 19.5 fmol ml(-1) in SW. The results presented demonstrate that changes in environmental salinity influences both synthesis of CNP from the heart and also circulating levels in C. leucas. Potential stimulus for release and modes of action are discussed. (c) 2005 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)90-98
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume144
Issue number1
DOIs
Publication statusPublished - Oct 2005

Keywords

  • euryhaline
  • bull shark
  • Carcharhinus
  • natriuretic peptide
  • RENIN-ANGIOTENSIN SYSTEM
  • RECTAL GLAND-FUNCTION
  • TROUT ONCORHYNCHUS-MYKISS
  • LESSER-SPOTTED DOGFISH
  • VOLUME EXPANSION
  • NEUROHYPOPHYSEAL HORMONES
  • MOLECULAR EVOLUTION
  • SQUALUS-ACANTHIAS
  • TRIAKIS-SCYLLIA
  • FRESH-WATER

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