Abstract
The blood filtration method was used as the gold standard to determine the detection level of simple blood-spot sampling and nested-polymerase chain reaction (PCR) for Brugia malayi. Of 100 samples, 48 were filtration-positive. Of these, 26 had microfilaria counts that were low enough (<1-29 microfilariae/ml) to accurately assess the limit of detection by nested-PCR. Nested-PCR consistently detected B. malayi DNA in samples with <greater than or equal to>10 microfilariae/ml, Post-filtration, microfilaria-depleted. blood-spots from microfilaria-positive samples were screened by nested-PCR and B. malayi specific 'free' DNA was detected in 51.7% of these samples. There was no evidence for 'free' DNA in microfilaria-negative individuals from this endemic community. (C) 2000 Published by Elsevier Science Ltd. on behalf of the Australian Society for Parasitology.
| Original language | English |
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| Pages (from-to) | 1177-1179 |
| Number of pages | 3 |
| Journal | International Journal for Parasitology |
| Volume | 30 |
| Issue number | 11 |
| Publication status | Published - Oct 2000 |