Segment-specific optogenetic stimulation in Drosophila melanogaster with linear arrays of organic light-emitting diodes

Caroline Murawski; Stefan Robert Pulver; Malte C. Gather

doi:10.1038/s41467-020-20013-6

Segment-specific optogenetic stimulation in Drosophila melanogaster with linear arrays of organic light-emitting diodes

Caroline Murawski, Stefan Robert Pulver, Malte C. Gather

Research output: Contribution to journal › Article › peer-review

Abstract

Optogenetics allows light-driven, non-contact control of neural systems, but light delivery remains challenging, in particular when fine spatial control of light is required to achieve local specificity. Here, we employ organic light-emitting diodes (OLEDs) that are micropatterned into linear arrays to obtain precise optogenetic control in Drosophila melanogaster larvae expressing the light-gated activator CsChrimson and the inhibitor GtACR2 within their peripheral sensory system. Our method allows confinement of light stimuli to within individual abdominal segments, which facilitates the study of larval behaviour in response to local sensory input. We show controlled triggering of specific crawling modes and find that targeted neurostimulation in abdominal segments switches the direction of crawling. More broadly, our work demonstrates how OLEDs can provide tailored patterns of light for photo-stimulation of neuronal networks, with future implications ranging from mapping neuronal connectivity in cultures to targeted photo-stimulation with pixelated OLED implants in vivo.

Original language	English
Article number	6248
Number of pages	11
Journal	Nature Communications
Volume	11
DOIs	https://doi.org/10.1038/s41467-020-20013-6
Publication status	Published - 7 Dec 2020

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Cite this

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title = "Segment-specific optogenetic stimulation in Drosophila melanogaster with linear arrays of organic light-emitting diodes",

abstract = "Optogenetics allows light-driven, non-contact control of neural systems, but light delivery remains challenging, in particular when fine spatial control of light is required to achieve local specificity. Here, we employ organic light-emitting diodes (OLEDs) that are micropatterned into linear arrays to obtain precise optogenetic control in Drosophila melanogaster larvae expressing the light-gated activator CsChrimson and the inhibitor GtACR2 within their peripheral sensory system. Our method allows confinement of light stimuli to within individual abdominal segments, which facilitates the study of larval behaviour in response to local sensory input. We show controlled triggering of specific crawling modes and find that targeted neurostimulation in abdominal segments switches the direction of crawling. More broadly, our work demonstrates how OLEDs can provide tailored patterns of light for photo-stimulation of neuronal networks, with future implications ranging from mapping neuronal connectivity in cultures to targeted photo-stimulation with pixelated OLED implants in vivo.",

author = "Caroline Murawski and Pulver, {Stefan Robert} and Gather, {Malte C.}",

note = "This research was financially supported by the EPSRC NSF-CBET lead agency agreement (EP/R010595/1, 1706207), the DARPA-NESD programme (N66001-17-C-4012) and the Leverhulme Trust (RPG-2017-231). C.M. acknowledges funding from the European Commission through a Marie Sk{\l}odowska Curie individual fellowship (703387). S.R.P acknowledges funding from the Biology and Biotechnology Research council (BB/M021793). M.C.G. acknowledges funding from the Alexander von Humboldt Stiftung (Humboldt-Professorship). ",

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doi = "10.1038/s41467-020-20013-6",

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journal = "Nature Communications",

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AU - Murawski, Caroline

AU - Pulver, Stefan Robert

AU - Gather, Malte C.

N1 - This research was financially supported by the EPSRC NSF-CBET lead agency agreement (EP/R010595/1, 1706207), the DARPA-NESD programme (N66001-17-C-4012) and the Leverhulme Trust (RPG-2017-231). C.M. acknowledges funding from the European Commission through a Marie Skłodowska Curie individual fellowship (703387). S.R.P acknowledges funding from the Biology and Biotechnology Research council (BB/M021793). M.C.G. acknowledges funding from the Alexander von Humboldt Stiftung (Humboldt-Professorship).

PY - 2020/12/7

Y1 - 2020/12/7

N2 - Optogenetics allows light-driven, non-contact control of neural systems, but light delivery remains challenging, in particular when fine spatial control of light is required to achieve local specificity. Here, we employ organic light-emitting diodes (OLEDs) that are micropatterned into linear arrays to obtain precise optogenetic control in Drosophila melanogaster larvae expressing the light-gated activator CsChrimson and the inhibitor GtACR2 within their peripheral sensory system. Our method allows confinement of light stimuli to within individual abdominal segments, which facilitates the study of larval behaviour in response to local sensory input. We show controlled triggering of specific crawling modes and find that targeted neurostimulation in abdominal segments switches the direction of crawling. More broadly, our work demonstrates how OLEDs can provide tailored patterns of light for photo-stimulation of neuronal networks, with future implications ranging from mapping neuronal connectivity in cultures to targeted photo-stimulation with pixelated OLED implants in vivo.

AB - Optogenetics allows light-driven, non-contact control of neural systems, but light delivery remains challenging, in particular when fine spatial control of light is required to achieve local specificity. Here, we employ organic light-emitting diodes (OLEDs) that are micropatterned into linear arrays to obtain precise optogenetic control in Drosophila melanogaster larvae expressing the light-gated activator CsChrimson and the inhibitor GtACR2 within their peripheral sensory system. Our method allows confinement of light stimuli to within individual abdominal segments, which facilitates the study of larval behaviour in response to local sensory input. We show controlled triggering of specific crawling modes and find that targeted neurostimulation in abdominal segments switches the direction of crawling. More broadly, our work demonstrates how OLEDs can provide tailored patterns of light for photo-stimulation of neuronal networks, with future implications ranging from mapping neuronal connectivity in cultures to targeted photo-stimulation with pixelated OLED implants in vivo.

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DO - 10.1038/s41467-020-20013-6

M3 - Article

SN - 2041-1723

VL - 11

JO - Nature Communications

JF - Nature Communications

M1 - 6248

ER -

Segment-specific optogenetic stimulation in Drosophila melanogaster with linear arrays of organic light-emitting diodes

Abstract

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H2020 MSCA Fellowship NeurOLED: H2020 MSCA Fellowship (Caroline Murawski)

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