SARS-CoV-2 multi-antigen serology assay

Ramin Mazhari; Shazia Ruybal-Pesántez; Fiona Angrisano; Nicholas Kiernan-Walker; Stephanie Hyslop; Rhea J. Longley; Caitlin Bourke; Catherine Chen; Deborah A. Williamson; Leanne J. Robinson; Ivo Mueller; Emily M. Eriksson

doi:10.3390/mps4040072

SARS-CoV-2 multi-antigen serology assay

Ramin Mazhari, Shazia Ruybal-Pesántez, Fiona Angrisano, Nicholas Kiernan-Walker, Stephanie Hyslop, Rhea J. Longley, Caitlin Bourke, Catherine Chen, Deborah A. Williamson, Leanne J. Robinson, Ivo Mueller, Emily M. Eriksson^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Serology tests are extremely useful for assessing whether a person has been infected with a pathogen. Since the onset of the COVID-19 pandemic, measurement of anti-SARS-CoV-2-specific antibodies has been considered an essential tool in identifying seropositive individuals and thereby understanding the extent of transmission in communities. The Luminex system is a bead-based technology that has the capacity to assess multiple antigens simultaneously using very low sample volumes and is ideal for high-throughput studies. We have adapted this technology to develop a COVID-19 multi-antigen serological assay. This protocol described here carefully outlines recommended steps to optimize and establish this method for COVID-19-specific antibody measurement in plasma and in saliva. However, the protocol can easily be customized and thus the assay is broadly applicable to measure antibodies to other pathogens.

Original language	English
Article number	72
Journal	Methods and Protocols
Volume	4
Issue number	4
DOIs	https://doi.org/10.3390/mps4040072
Publication status	Published - 9 Oct 2021

Keywords

COVID-19
Luminex
SARS-CoV-2
Serology

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10.3390/mps4040072Licence: CC BY

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title = "SARS-CoV-2 multi-antigen serology assay",

abstract = "Serology tests are extremely useful for assessing whether a person has been infected with a pathogen. Since the onset of the COVID-19 pandemic, measurement of anti-SARS-CoV-2-specific antibodies has been considered an essential tool in identifying seropositive individuals and thereby understanding the extent of transmission in communities. The Luminex system is a bead-based technology that has the capacity to assess multiple antigens simultaneously using very low sample volumes and is ideal for high-throughput studies. We have adapted this technology to develop a COVID-19 multi-antigen serological assay. This protocol described here carefully outlines recommended steps to optimize and establish this method for COVID-19-specific antibody measurement in plasma and in saliva. However, the protocol can easily be customized and thus the assay is broadly applicable to measure antibodies to other pathogens.",

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author = "Ramin Mazhari and Shazia Ruybal-Pes{\'a}ntez and Fiona Angrisano and Nicholas Kiernan-Walker and Stephanie Hyslop and Longley, \{Rhea J.\} and Caitlin Bourke and Catherine Chen and Williamson, \{Deborah A.\} and Robinson, \{Leanne J.\} and Ivo Mueller and Eriksson, \{Emily M.\}",

note = "This work was supported by WHO Unity funds and WEHI philanthropic funds. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. L.J.R. and I.M. are supported by NHMRC Research Fellowships (GNT1161627 and GNT1043345). R.J.L. is supported by a NHMRC Early Leadership Fellowship (\#1173210).",

year = "2021",

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doi = "10.3390/mps4040072",

language = "English",

volume = "4",

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AU - Mazhari, Ramin

AU - Ruybal-Pesántez, Shazia

AU - Angrisano, Fiona

AU - Kiernan-Walker, Nicholas

AU - Hyslop, Stephanie

AU - Longley, Rhea J.

AU - Bourke, Caitlin

AU - Chen, Catherine

AU - Williamson, Deborah A.

AU - Robinson, Leanne J.

AU - Mueller, Ivo

AU - Eriksson, Emily M.

N1 - This work was supported by WHO Unity funds and WEHI philanthropic funds. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. L.J.R. and I.M. are supported by NHMRC Research Fellowships (GNT1161627 and GNT1043345). R.J.L. is supported by a NHMRC Early Leadership Fellowship (#1173210).

PY - 2021/10/9

Y1 - 2021/10/9

N2 - Serology tests are extremely useful for assessing whether a person has been infected with a pathogen. Since the onset of the COVID-19 pandemic, measurement of anti-SARS-CoV-2-specific antibodies has been considered an essential tool in identifying seropositive individuals and thereby understanding the extent of transmission in communities. The Luminex system is a bead-based technology that has the capacity to assess multiple antigens simultaneously using very low sample volumes and is ideal for high-throughput studies. We have adapted this technology to develop a COVID-19 multi-antigen serological assay. This protocol described here carefully outlines recommended steps to optimize and establish this method for COVID-19-specific antibody measurement in plasma and in saliva. However, the protocol can easily be customized and thus the assay is broadly applicable to measure antibodies to other pathogens.

AB - Serology tests are extremely useful for assessing whether a person has been infected with a pathogen. Since the onset of the COVID-19 pandemic, measurement of anti-SARS-CoV-2-specific antibodies has been considered an essential tool in identifying seropositive individuals and thereby understanding the extent of transmission in communities. The Luminex system is a bead-based technology that has the capacity to assess multiple antigens simultaneously using very low sample volumes and is ideal for high-throughput studies. We have adapted this technology to develop a COVID-19 multi-antigen serological assay. This protocol described here carefully outlines recommended steps to optimize and establish this method for COVID-19-specific antibody measurement in plasma and in saliva. However, the protocol can easily be customized and thus the assay is broadly applicable to measure antibodies to other pathogens.

KW - COVID-19

KW - Luminex

KW - SARS-CoV-2

KW - Serology

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DO - 10.3390/mps4040072

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VL - 4

JO - Methods and Protocols

JF - Methods and Protocols

IS - 4

M1 - 72

ER -

SARS-CoV-2 multi-antigen serology assay

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