S100A4 (p9Ka) protein in colon carcinoma and liver metastases: association with carcinoma cells and T-lymphocytes

S Taylor, Charles Simon Herrington, W Prime, PS Rudland, R Barraclough

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

The presence of the EF-hand-calcium-binding protein S 100A4 in the carcinoma cells of the primary tumour is associated with a shorter survival time of a group of breast cancer patients. In colon cancer, primary tumours as well as metastases to the liver can be studied. Here we show, using quantitative PCR applied to RNA from 24 normal colon, four liver tissues. 24 colon carcinoma specimens, and 24 livers containing colonic carcinoma metastases, that the level of S 100A4 mRNA was significantly higher in the carcinomas compared to normal specimers (Mann-Whitney U-test, P=0.05), and in liver metastases compared to carcinoma specimens (P=0.039). The latter comparison included seven liver metastases and their matched primary carcinomas (P < 0.001) from the same patient. In situ hybridization and immunocytochemistry techniques have localized S 100A4 to both carcinoma cells and lymphocytes in the malignant specimens. The percentage of specimens stained for S 100A4 in the epithelial cells is significantly higher for those isolated from carcinomas and metastases than from the corresponding normal tissue, and from metastases than from corresponding carcinoma (Fisher Exact text, P < 0.0016, P=0.04, respectively). In most specimens, S 100A4 is present in clusters of T lymphocytes and this distribution is also found in the lymphoid, uninflamed appendix.

Original languageEnglish
Pages (from-to)409-416
Number of pages8
JournalBritish Journal of Cancer
Volume86
Issue number3
DOIs
Publication statusPublished - 1 Feb 2002

Keywords

  • p9Ka
  • S100A4
  • T-lymphocytes
  • colon carcinoma
  • metastasis
  • CALCIUM-BINDING PROTEIN
  • BREAST-CANCER SPECIMENS
  • C-ERBB-2 MESSENGER-RNA
  • MTS1 GENE
  • EXPRESSION
  • PHENOTYPE
  • LINE

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