RNA sequence analysis of diseased groundnut (Arachis hypogaea) reveals the full genome of groundnut rosette assistor virus (GRAV)

Susan Jones; Graham Cowan; Stuart MacFarlane; Benard Mukoye; Bonphace Collins Mangeni; Hassan Were; Lesley Torrance

doi:10.1016/j.virusres.2019.197837

RNA sequence analysis of diseased groundnut (Arachis hypogaea) reveals the full genome of groundnut rosette assistor virus (GRAV)

Susan Jones, Graham Cowan, Stuart MacFarlane, Benard Mukoye, Bonphace Collins Mangeni, Hassan Were, Lesley Torrance

Research output: Contribution to journal › Article › peer-review

Abstract

The complete genome sequences for two variant isolates of groundnut rosette assistor virus (GRAV) have been determined from symptomatic groundnut plants in western Kenya. The sequences of the two GRAV isolates (sc7.1 and sc7.2) are 84.2% identical at the nucleotide level and 98.5% identical at the coat protein level. The variants sc7.1 and sc7.2 comprise 5850 and 5879 nucleotides respectively, and show similar genome organizations with 7 predicted ORFs (P0, P1, P2, P3a, P3 (coat protein, CP), P4 (movement protein, MP) and P5 (coat protein-readthrough protein, CP-RT). Currently, GRAV is an unassigned virus in the Luteoviridae family, due to the fact that only the sequence of the coat protein was previously obtained. The presence of both ORF0 and ORF 4 within the genome sequence determined in the current work suggest that GRAV should be classified as a member of the genus Polerovirus.

Original language	English
Article number	197837
Journal	Virus Research
Volume	277
Early online date	10 Dec 2019
DOIs	https://doi.org/10.1016/j.virusres.2019.197837
Publication status	Published - Feb 2020

Keywords

RNA-sequencing
Groundnut
Groundnut rosette assistor virus
Polerovirus
Luteovirus

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10.1016/j.virusres.2019.197837

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@article{18d7ddd9a7674bfba41fe86e6bcf0869,

title = "RNA sequence analysis of diseased groundnut (Arachis hypogaea) reveals the full genome of groundnut rosette assistor virus (GRAV)",

abstract = "The complete genome sequences for two variant isolates of groundnut rosette assistor virus (GRAV) have been determined from symptomatic groundnut plants in western Kenya. The sequences of the two GRAV isolates (sc7.1 and sc7.2) are 84.2% identical at the nucleotide level and 98.5% identical at the coat protein level. The variants sc7.1 and sc7.2 comprise 5850 and 5879 nucleotides respectively, and show similar genome organizations with 7 predicted ORFs (P0, P1, P2, P3a, P3 (coat protein, CP), P4 (movement protein, MP) and P5 (coat protein-readthrough protein, CP-RT). Currently, GRAV is an unassigned virus in the Luteoviridae family, due to the fact that only the sequence of the coat protein was previously obtained. The presence of both ORF0 and ORF 4 within the genome sequence determined in the current work suggest that GRAV should be classified as a member of the genus Polerovirus.",

keywords = "RNA-sequencing, Groundnut, Groundnut rosette assistor virus, Polerovirus, Luteovirus",

author = "Susan Jones and Graham Cowan and Stuart MacFarlane and Benard Mukoye and Mangeni, {Bonphace Collins} and Hassan Were and Lesley Torrance",

note = "This work was supported by a Royal Society Africa Exchange Grant [reference SM150075] “Strengthening crop disease diagnostic capabilities in Kenya”. The work of SJ, SM, GC and LT was additionally supported by the Scottish Government{\textquoteright}s Rural and Environment Science and Analytical Services division (RESAS).",

year = "2020",

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doi = "10.1016/j.virusres.2019.197837",

language = "English",

volume = "277",

journal = "Virus Research",

issn = "0168-1702",

publisher = "Elsevier",

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T1 - RNA sequence analysis of diseased groundnut (Arachis hypogaea) reveals the full genome of groundnut rosette assistor virus (GRAV)

AU - Jones, Susan

AU - Cowan, Graham

AU - MacFarlane, Stuart

AU - Mukoye, Benard

AU - Mangeni, Bonphace Collins

AU - Were, Hassan

AU - Torrance, Lesley

N1 - This work was supported by a Royal Society Africa Exchange Grant [reference SM150075] “Strengthening crop disease diagnostic capabilities in Kenya”. The work of SJ, SM, GC and LT was additionally supported by the Scottish Government’s Rural and Environment Science and Analytical Services division (RESAS).

PY - 2020/2

Y1 - 2020/2

N2 - The complete genome sequences for two variant isolates of groundnut rosette assistor virus (GRAV) have been determined from symptomatic groundnut plants in western Kenya. The sequences of the two GRAV isolates (sc7.1 and sc7.2) are 84.2% identical at the nucleotide level and 98.5% identical at the coat protein level. The variants sc7.1 and sc7.2 comprise 5850 and 5879 nucleotides respectively, and show similar genome organizations with 7 predicted ORFs (P0, P1, P2, P3a, P3 (coat protein, CP), P4 (movement protein, MP) and P5 (coat protein-readthrough protein, CP-RT). Currently, GRAV is an unassigned virus in the Luteoviridae family, due to the fact that only the sequence of the coat protein was previously obtained. The presence of both ORF0 and ORF 4 within the genome sequence determined in the current work suggest that GRAV should be classified as a member of the genus Polerovirus.

AB - The complete genome sequences for two variant isolates of groundnut rosette assistor virus (GRAV) have been determined from symptomatic groundnut plants in western Kenya. The sequences of the two GRAV isolates (sc7.1 and sc7.2) are 84.2% identical at the nucleotide level and 98.5% identical at the coat protein level. The variants sc7.1 and sc7.2 comprise 5850 and 5879 nucleotides respectively, and show similar genome organizations with 7 predicted ORFs (P0, P1, P2, P3a, P3 (coat protein, CP), P4 (movement protein, MP) and P5 (coat protein-readthrough protein, CP-RT). Currently, GRAV is an unassigned virus in the Luteoviridae family, due to the fact that only the sequence of the coat protein was previously obtained. The presence of both ORF0 and ORF 4 within the genome sequence determined in the current work suggest that GRAV should be classified as a member of the genus Polerovirus.

KW - RNA-sequencing

KW - Groundnut

KW - Groundnut rosette assistor virus

KW - Polerovirus

KW - Luteovirus

U2 - 10.1016/j.virusres.2019.197837

DO - 10.1016/j.virusres.2019.197837

M3 - Article

C2 - 31836513

SN - 0168-1702

VL - 277

JO - Virus Research

JF - Virus Research

M1 - 197837

ER -

RNA sequence analysis of diseased groundnut (Arachis hypogaea) reveals the full genome of groundnut rosette assistor virus (GRAV)

Abstract

Keywords

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