RNA Polymerase I-mediated expression of viral RNA for the rescue of infectious virulent and avirulent Rift Valley fever viruses

A Billecocoq; N Gauliard; N Le May; Richard Michael Elliott; R Flick; M Bouloy

doi:10.1016/j.virol.2008.05.033

RNA Polymerase I-mediated expression of viral RNA for the rescue of infectious virulent and avirulent Rift Valley fever viruses

A Billecocoq, N Gauliard, N Le May, Richard Michael Elliott, R Flick, M Bouloy

Research output: Contribution to journal › Article › peer-review

Abstract

Rift Valley fever Virus (RVFV, Bunyaviridae, Phlebovirtis) is a mosquito-transmitted arbovirus that causes human and animal diseases in sub-Saharan Africa and was introduced into tire Arabian Peninsula ill 2000. Here, we describe a method of reverse genetics to recover infectious RVFV from transfected plasmids based Oil the use Of the cellular RNA polymerase I promoter to synthesize viral transcripts. We compared its efficiency with a system using T7 RNA polymerase and found that both are equally efficient for the rescue of RVFV generating titers of approx. 10(7) to 10(8) pfu/ml. We used the RNA polymerase 1-based system to rescue both attenuated MP12 and virulent ZH548 strains as well as chimeric MP12-ZH548 viruses, and in addition RVFV expressing reporter proteins. (C) 2008 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	377-384
Number of pages	8
Journal	Virology
Volume	378
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2008.05.033
Publication status	Published - 1 Sept 2008

Keywords

bunyavirus
phlebovirus
Rift Valley fever virus
nonstructural protein NSs
expression of reporter protein
reverse genetics
RNA polymerase 1
REVERSE-GENETICS SYSTEM
NONSTRUCTURAL PROTEIN NSS
CLONED CDNA
FOREIGN GENE
POLYMERASE-I
LACKING
GENOME
BUNYAVIRIDAE
ATTENUATION
TRANSCRIPTION

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2008.05.033

Cite this

@article{b0ee592b241f46df8e09cb6ed66b298a,

title = "RNA Polymerase I-mediated expression of viral RNA for the rescue of infectious virulent and avirulent Rift Valley fever viruses",

abstract = "Rift Valley fever Virus (RVFV, Bunyaviridae, Phlebovirtis) is a mosquito-transmitted arbovirus that causes human and animal diseases in sub-Saharan Africa and was introduced into tire Arabian Peninsula ill 2000. Here, we describe a method of reverse genetics to recover infectious RVFV from transfected plasmids based Oil the use Of the cellular RNA polymerase I promoter to synthesize viral transcripts. We compared its efficiency with a system using T7 RNA polymerase and found that both are equally efficient for the rescue of RVFV generating titers of approx. 10(7) to 10(8) pfu/ml. We used the RNA polymerase 1-based system to rescue both attenuated MP12 and virulent ZH548 strains as well as chimeric MP12-ZH548 viruses, and in addition RVFV expressing reporter proteins. (C) 2008 Elsevier Inc. All rights reserved.",

keywords = "bunyavirus, phlebovirus, Rift Valley fever virus, nonstructural protein NSs, expression of reporter protein, reverse genetics, RNA polymerase 1, REVERSE-GENETICS SYSTEM, NONSTRUCTURAL PROTEIN NSS, CLONED CDNA, FOREIGN GENE, POLYMERASE-I, LACKING, GENOME, BUNYAVIRIDAE, ATTENUATION, TRANSCRIPTION",

author = "A Billecocoq and N Gauliard and {Le May}, N and Elliott, {Richard Michael} and R Flick and M Bouloy",

year = "2008",

month = sep,

day = "1",

doi = "10.1016/j.virol.2008.05.033",

language = "English",

volume = "378",

pages = "377--384",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press/Elsevier ",

number = "2",

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TY - JOUR

T1 - RNA Polymerase I-mediated expression of viral RNA for the rescue of infectious virulent and avirulent Rift Valley fever viruses

AU - Billecocoq, A

AU - Gauliard, N

AU - Le May, N

AU - Elliott, Richard Michael

AU - Flick, R

AU - Bouloy, M

PY - 2008/9/1

Y1 - 2008/9/1

N2 - Rift Valley fever Virus (RVFV, Bunyaviridae, Phlebovirtis) is a mosquito-transmitted arbovirus that causes human and animal diseases in sub-Saharan Africa and was introduced into tire Arabian Peninsula ill 2000. Here, we describe a method of reverse genetics to recover infectious RVFV from transfected plasmids based Oil the use Of the cellular RNA polymerase I promoter to synthesize viral transcripts. We compared its efficiency with a system using T7 RNA polymerase and found that both are equally efficient for the rescue of RVFV generating titers of approx. 10(7) to 10(8) pfu/ml. We used the RNA polymerase 1-based system to rescue both attenuated MP12 and virulent ZH548 strains as well as chimeric MP12-ZH548 viruses, and in addition RVFV expressing reporter proteins. (C) 2008 Elsevier Inc. All rights reserved.

AB - Rift Valley fever Virus (RVFV, Bunyaviridae, Phlebovirtis) is a mosquito-transmitted arbovirus that causes human and animal diseases in sub-Saharan Africa and was introduced into tire Arabian Peninsula ill 2000. Here, we describe a method of reverse genetics to recover infectious RVFV from transfected plasmids based Oil the use Of the cellular RNA polymerase I promoter to synthesize viral transcripts. We compared its efficiency with a system using T7 RNA polymerase and found that both are equally efficient for the rescue of RVFV generating titers of approx. 10(7) to 10(8) pfu/ml. We used the RNA polymerase 1-based system to rescue both attenuated MP12 and virulent ZH548 strains as well as chimeric MP12-ZH548 viruses, and in addition RVFV expressing reporter proteins. (C) 2008 Elsevier Inc. All rights reserved.

KW - bunyavirus

KW - phlebovirus

KW - Rift Valley fever virus

KW - nonstructural protein NSs

KW - expression of reporter protein

KW - reverse genetics

KW - RNA polymerase 1

KW - REVERSE-GENETICS SYSTEM

KW - NONSTRUCTURAL PROTEIN NSS

KW - CLONED CDNA

KW - FOREIGN GENE

KW - POLYMERASE-I

KW - LACKING

KW - GENOME

KW - BUNYAVIRIDAE

KW - ATTENUATION

KW - TRANSCRIPTION

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U2 - 10.1016/j.virol.2008.05.033

DO - 10.1016/j.virol.2008.05.033

M3 - Article

SN - 0042-6822

VL - 378

SP - 377

EP - 384

JO - Virology

JF - Virology

IS - 2

ER -

RNA Polymerase I-mediated expression of viral RNA for the rescue of infectious virulent and avirulent Rift Valley fever viruses

Abstract

Keywords

UN SDGs

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Wellcome Trust 079810/Z/06/Z: Molecular biology of bunyavirus host cell interactions

Cite this

RNA Polymerase I-mediated expression of viral RNA for the rescue of infectious virulent and avirulent Rift Valley fever viruses

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

Wellcome Trust 079810/Z/06/Z: Molecular biology of bunyavirus host cell interactions

Cite this