Ribosomal intergenic spacer: A polymerase chain reaction diagnostic for Meloidogyne chitwoodi, M-fallax, and M-hapla

J Wishart, M S Phillips, V C Blok

Research output: Contribution to journalArticlepeer-review

79 Citations (Scopus)

Abstract

Polymerase chain reaction amplification of the intergenic spacer region between the 5S and 18S genes from Meloidogyne chitwoodi. M. fallax, and M. hapla enabled these three important temperate species to be differentiated. Length polymorphism was found between M. chitwoodi and M. fallax as a result of differing numbers of short repeats located between the 5S and 18S genes. The presence of the 5S gene within the rDNA cistrons was confirmed in the Meloidogyne spp. included in this study. The region between the 28S and 5S genes for M. chitwoodi and M. fallax was and lacked variability in repeated sequences compared with the main tropical Meloidogyne spp. and M. hapla. Differences in the number of these repeats resulted in intraspecific length polymorphism for M. hapla.

Original languageEnglish
Pages (from-to)884-892
Number of pages9
JournalPhytopathology
Volume92
Issue number8
DOIs
Publication statusPublished - Aug 2002

Keywords

  • repeated DNA sequences
  • root-knot nematodes
  • ROOT-KNOT NEMATODES
  • DNA REPEATS
  • PCR ASSAY
  • ARENARIA
  • IDENTIFICATION
  • SEQUENCES
  • HETEROGENEITY
  • DIFFERENTIATE
  • EVOLUTION
  • REGION

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