Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in northern Tanzania

S. H. Gillespie; N. Kennedy; F. I. Ngowi; N. G. Fomukong; S. Al-Maamary; J. W. Dale

doi:10.1016/0035-9203(95)90571-5

Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in northern Tanzania

S. H. Gillespie^*
, N. Kennedy
, F. I. Ngowi
, N. G. Fomukong
, S. Al-Maamary
, J. W. Dale

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Isolates of Mycobacterium tuberculosis from 88 patients with pulmonary tuberculosis in northern Tanzania were subjected to IS6110 restriction fragment length polymorphism (RFLP) analysis. Of 88 isolates, 73 fell into 11 groups of which 9 contained 2-5 isolates. Of 2 large homology groupsone, group H (20 isolates), was isolated only from patients resident in the Kilimanjiro region, whereas 79% of isolates from other groups came from this region. A significant association (P=0.023) was found between another group, M (24 isolates) and isolation from patients of the Masai tribe. The data from this pilot study support the idea that IS6110 RFLP analysisprovides information which may be of value in the control of tuberculosis in Africa.

Original language	English
Pages (from-to)	335-338
Number of pages	4
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	89
Issue number	3
DOIs	https://doi.org/10.1016/0035-9203(95)90571-5
Publication status	Published - 1 Jan 1995

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Mycobacterium tuberculosis
Restriction fragment length polymorphism analysis
Strain relationships
Tanzania
Tuberculosis

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Gillespie, S. H., Kennedy, N., Ngowi, F. I., Fomukong, N. G., Al-Maamary, S., & Dale, J. W. (1995). Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in northern Tanzania. Transactions of the Royal Society of Tropical Medicine and Hygiene, 89(3), 335-338. https://doi.org/10.1016/0035-9203(95)90571-5

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title = "Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in northern Tanzania",

abstract = "Isolates of Mycobacterium tuberculosis from 88 patients with pulmonary tuberculosis in northern Tanzania were subjected to IS6110 restriction fragment length polymorphism (RFLP) analysis. Of 88 isolates, 73 fell into 11 groups of which 9 contained 2-5 isolates. Of 2 large homology groupsone, group H (20 isolates), was isolated only from patients resident in the Kilimanjiro region, whereas 79\% of isolates from other groups came from this region. A significant association (P=0.023) was found between another group, M (24 isolates) and isolation from patients of the Masai tribe. The data from this pilot study support the idea that IS6110 RFLP analysisprovides information which may be of value in the control of tuberculosis in Africa.",

keywords = "Mycobacterium tuberculosis, Restriction fragment length polymorphism analysis, Strain relationships, Tanzania, Tuberculosis",

author = "Gillespie, \{S. H.\} and N. Kennedy and Ngowi, \{F. I.\} and Fomukong, \{N. G.\} and S. Al-Maamary and Dale, \{J. W.\}",

year = "1995",

month = jan,

day = "1",

doi = "10.1016/0035-9203(95)90571-5",

language = "English",

volume = "89",

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journal = "Transactions of the Royal Society of Tropical Medicine and Hygiene",

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Gillespie, SH, Kennedy, N, Ngowi, FI, Fomukong, NG, Al-Maamary, S & Dale, JW 1995, 'Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in northern Tanzania', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 89, no. 3, pp. 335-338. https://doi.org/10.1016/0035-9203(95)90571-5

Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in northern Tanzania. / Gillespie, S. H.; Kennedy, N.; Ngowi, F. I. et al.
In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 89, No. 3, 01.01.1995, p. 335-338.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in northern Tanzania

AU - Gillespie, S. H.

AU - Kennedy, N.

AU - Ngowi, F. I.

AU - Fomukong, N. G.

AU - Al-Maamary, S.

AU - Dale, J. W.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Isolates of Mycobacterium tuberculosis from 88 patients with pulmonary tuberculosis in northern Tanzania were subjected to IS6110 restriction fragment length polymorphism (RFLP) analysis. Of 88 isolates, 73 fell into 11 groups of which 9 contained 2-5 isolates. Of 2 large homology groupsone, group H (20 isolates), was isolated only from patients resident in the Kilimanjiro region, whereas 79% of isolates from other groups came from this region. A significant association (P=0.023) was found between another group, M (24 isolates) and isolation from patients of the Masai tribe. The data from this pilot study support the idea that IS6110 RFLP analysisprovides information which may be of value in the control of tuberculosis in Africa.

AB - Isolates of Mycobacterium tuberculosis from 88 patients with pulmonary tuberculosis in northern Tanzania were subjected to IS6110 restriction fragment length polymorphism (RFLP) analysis. Of 88 isolates, 73 fell into 11 groups of which 9 contained 2-5 isolates. Of 2 large homology groupsone, group H (20 isolates), was isolated only from patients resident in the Kilimanjiro region, whereas 79% of isolates from other groups came from this region. A significant association (P=0.023) was found between another group, M (24 isolates) and isolation from patients of the Masai tribe. The data from this pilot study support the idea that IS6110 RFLP analysisprovides information which may be of value in the control of tuberculosis in Africa.

KW - Mycobacterium tuberculosis

KW - Restriction fragment length polymorphism analysis

KW - Strain relationships

KW - Tanzania

KW - Tuberculosis

UR - https://www.scopus.com/pages/publications/0029041309

U2 - 10.1016/0035-9203(95)90571-5

DO - 10.1016/0035-9203(95)90571-5

M3 - Article

C2 - 7660455

AN - SCOPUS:0029041309

SN - 0035-9203

VL - 89

SP - 335

EP - 338

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 3

ER -

Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in northern Tanzania

Abstract

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Keywords

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