Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in northern Tanzania

S. H. Gillespie*, N. Kennedy, F. I. Ngowi, N. G. Fomukong, S. Al-Maamary, J. W. Dale

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Isolates of Mycobacterium tuberculosis from 88 patients with pulmonary tuberculosis in northern Tanzania were subjected to IS6110 restriction fragment length polymorphism (RFLP) analysis. Of 88 isolates, 73 fell into 11 groups of which 9 contained 2-5 isolates. Of 2 large homology groupsone, group H (20 isolates), was isolated only from patients resident in the Kilimanjiro region, whereas 79% of isolates from other groups came from this region. A significant association (P=0.023) was found between another group, M (24 isolates) and isolation from patients of the Masai tribe. The data from this pilot study support the idea that IS6110 RFLP analysisprovides information which may be of value in the control of tuberculosis in Africa.

Original languageEnglish
Pages (from-to)335-338
Number of pages4
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Volume89
Issue number3
DOIs
Publication statusPublished - 1 Jan 1995

Keywords

  • Mycobacterium tuberculosis
  • Restriction fragment length polymorphism analysis
  • Strain relationships
  • Tanzania
  • Tuberculosis

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