Reproducible surface-enhanced Raman quantification of biomarkers in multicomponent mixtures

Anna Chiara De Luca*, Peter Reader-Harris, Michael Mazilu, Stefania Mariggio, Daniela Corda, Andrea Di Falco

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)
1 Downloads (Pure)


Direct and quantitative detection of unlabeled glycerophosphoinositol (GroPIns), an abundant cytosolic phosphoinositide derivative, would allow rapid evaluation of several malignant cell transformations. Here we report label-free analysis of GroPIns via surface-enhanced Raman spectroscopy (SERS) with a sensitivity of 200 nM, well below its apparent concentration in cells. Crucially, our SERS substrates, based on lithographically defined gold nanofeatures, can be used to predict accurately the GroPIns concentration even in multicomponent mixtures, avoiding the preliminary separation of individual compounds. Our results represent a critical step toward the creation of SERS-based biosensor for rapid, label-free, and reproducible detection of specific molecules, overcoming limits of current experimental methods.

Original languageEnglish
Pages (from-to)2575-2583
Number of pages9
JournalACS Nano
Issue number3
Early online date13 Feb 2014
Publication statusPublished - 25 Mar 2014


  • SERS
  • Biosensor
  • Glycerophosphoinositol
  • Nanofabrication
  • Label-free detection
  • Tandem mass-spectrometry
  • Spectroscopy
  • Scattering
  • Substrate
  • Silver
  • Glycerophosphoinositols
  • Nanosensors
  • Spectra
  • Arrays


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