Regulation of neurotrophin receptor expression by retinoic acid in mouse sympathetic neuroblasts

S Wyatt, R Andres, H Rohrer, A M Davies

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

We have studied the effect of retinoic acid on the expression of the neurotrophin receptors trkA, trkC, and p75 by neuroblasts and neurons at different axial levels along the embryonic mouse paravertebral sympathetic chain. In dissociated cultures of sympathetic neuroblasts, retinoic acid inhibited the developmental increase in trkA mRNA expression and the developmental decrease in trkC mRNA expression that normally occurs in these cells but did not affect p75 mRNA expression. At higher concentrations, retinoic acid also increased the proliferation of sympathetic neuroblasts. After sympathetic neuroblasts became postmitotic, retinoic acid no longer affected receptor expression. Studies with retinoic acid receptor agonists and antagonists indicated that the effects of retinoic acid on neurotrophin receptor expression were mediated mainly by alpha retinoic acid receptors, not beta or gamma receptors. The observation that at-antagonists increased trkA mRNA expression in intact sympathetic ganglion explants suggests that endogenous retinoic acid is a physiological regulator of trkA receptor expression.

Original languageEnglish
Pages (from-to)1062-1071
Number of pages10
JournalThe Journal of Neuroscience
Volume19
Publication statusPublished - 1 Feb 1999

Keywords

  • retinoic acid
  • Trk receptor
  • p75 receptor
  • neurotrophin
  • sympathetic neuron
  • sympathetic neuroblast
  • NERVE GROWTH-FACTOR
  • TRIGEMINAL SENSORY NEURONS
  • TARGET FIELD INNERVATION
  • AFFINITY NGF RECEPTOR
  • PRECURSOR CELLS
  • MESSENGER-RNA
  • RAT TRKC
  • SURVIVAL
  • DIFFERENTIATION
  • DEATH

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