Reflex detection of ciprofloxacin resistance in Neisseria gonorrhoeae by use of the SpeeDx ResistancePlus GC assay

Darren Y.J. Lee; Melinda M. Ashcroft; Eric P.F. Chow; Michelle Sait; Vesna De Petra; Marlene Tschaepe; Sigrid Lange; George Taiaroa; Catriona S. Bradshaw; David M. Whiley; Christopher K. Fairley; Benjamin P. Howden; Marcus Y. Chen; Shivani Pasricha; Deborah A. Williamson

doi:10.1128/JCM.00089-21

Reflex detection of ciprofloxacin resistance in Neisseria gonorrhoeae by use of the SpeeDx ResistancePlus GC assay

Darren Y.J. Lee, Melinda M. Ashcroft, Eric P.F. Chow, Michelle Sait, Vesna De Petra, Marlene Tschaepe, Sigrid Lange, George Taiaroa, Catriona S. Bradshaw, David M. Whiley, Christopher K. Fairley, Benjamin P. Howden, Marcus Y. Chen, Shivani Pasricha, Deborah A. Williamson^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrhoeae-positive samples, 86.8% were found to possess the gyrA S91F mutation, which was highly predictive for ciprofloxacin resistance and stable across 3,144 publicly available N. gonorrhoeae genomes. Our work supports the feasibility of implementing RGT for gonorrhea into routine molecular workflows.

Original language	English
Article number	e00089-21
Journal	Journal of Clinical Microbiology
Volume	59
Issue number	5
DOIs	https://doi.org/10.1128/JCM.00089-21
Publication status	Published - 20 Apr 2021

Keywords

Antimicrobial resistance
Molecular diagnostic
Neisseria gonorrhoeae
Whole-genome sequencing

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Lee, D. Y. J., Ashcroft, M. M., Chow, E. P. F., Sait, M., De Petra, V., Tschaepe, M., Lange, S., Taiaroa, G., Bradshaw, C. S., Whiley, D. M., Fairley, C. K., Howden, B. P., Chen, M. Y., Pasricha, S., & Williamson, D. A. (2021). Reflex detection of ciprofloxacin resistance in Neisseria gonorrhoeae by use of the SpeeDx ResistancePlus GC assay. Journal of Clinical Microbiology, 59(5), Article e00089-21. https://doi.org/10.1128/JCM.00089-21

@article{8b25d2c619ab4bd18e66061d4d26573f,

title = "Reflex detection of ciprofloxacin resistance in Neisseria gonorrhoeae by use of the SpeeDx ResistancePlus GC assay",

abstract = "Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrhoeae-positive samples, 86.8% were found to possess the gyrA S91F mutation, which was highly predictive for ciprofloxacin resistance and stable across 3,144 publicly available N. gonorrhoeae genomes. Our work supports the feasibility of implementing RGT for gonorrhea into routine molecular workflows.",

keywords = "Antimicrobial resistance, Molecular diagnostic, Neisseria gonorrhoeae, Whole-genome sequencing",

author = "Lee, {Darren Y.J.} and Ashcroft, {Melinda M.} and Chow, {Eric P.F.} and Michelle Sait and {De Petra}, Vesna and Marlene Tschaepe and Sigrid Lange and George Taiaroa and Bradshaw, {Catriona S.} and Whiley, {David M.} and Fairley, {Christopher K.} and Howden, {Benjamin P.} and Chen, {Marcus Y.} and Shivani Pasricha and Williamson, {Deborah A.}",

note = "This study was supported by a Project Grant from the National Health and Medical Research Council (NHMRC) of Australia (GNT1147735 to D.A.W., M.Y.C., E.P.F.C., and C.K.F.). E.P.F.C. and D.A.W. are supported by an Australian National Health and Medical Research Council Emerging Leadership Investigator Grants (GNT1172873 and GNT1174555, respectively). C.K.F. and C.S.B. are supported by an NHMRC Leadership Investigator Grant (GNT1172900 and GNT1173361, respectively). B.P.H. is supported by an NHMRC Practitioner Fellowship (GNT1105905).",

year = "2021",

month = apr,

day = "20",

doi = "10.1128/JCM.00089-21",

language = "English",

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Lee, DYJ, Ashcroft, MM, Chow, EPF, Sait, M, De Petra, V, Tschaepe, M, Lange, S, Taiaroa, G, Bradshaw, CS, Whiley, DM, Fairley, CK, Howden, BP, Chen, MY, Pasricha, S & Williamson, DA 2021, 'Reflex detection of ciprofloxacin resistance in Neisseria gonorrhoeae by use of the SpeeDx ResistancePlus GC assay', Journal of Clinical Microbiology, vol. 59, no. 5, e00089-21. https://doi.org/10.1128/JCM.00089-21

TY - JOUR

T1 - Reflex detection of ciprofloxacin resistance in Neisseria gonorrhoeae by use of the SpeeDx ResistancePlus GC assay

AU - Lee, Darren Y.J.

AU - Ashcroft, Melinda M.

AU - Chow, Eric P.F.

AU - Sait, Michelle

AU - De Petra, Vesna

AU - Tschaepe, Marlene

AU - Lange, Sigrid

AU - Taiaroa, George

AU - Bradshaw, Catriona S.

AU - Whiley, David M.

AU - Fairley, Christopher K.

AU - Howden, Benjamin P.

AU - Chen, Marcus Y.

AU - Pasricha, Shivani

AU - Williamson, Deborah A.

N1 - This study was supported by a Project Grant from the National Health and Medical Research Council (NHMRC) of Australia (GNT1147735 to D.A.W., M.Y.C., E.P.F.C., and C.K.F.). E.P.F.C. and D.A.W. are supported by an Australian National Health and Medical Research Council Emerging Leadership Investigator Grants (GNT1172873 and GNT1174555, respectively). C.K.F. and C.S.B. are supported by an NHMRC Leadership Investigator Grant (GNT1172900 and GNT1173361, respectively). B.P.H. is supported by an NHMRC Practitioner Fellowship (GNT1105905).

PY - 2021/4/20

Y1 - 2021/4/20

N2 - Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrhoeae-positive samples, 86.8% were found to possess the gyrA S91F mutation, which was highly predictive for ciprofloxacin resistance and stable across 3,144 publicly available N. gonorrhoeae genomes. Our work supports the feasibility of implementing RGT for gonorrhea into routine molecular workflows.

AB - Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrhoeae-positive samples, 86.8% were found to possess the gyrA S91F mutation, which was highly predictive for ciprofloxacin resistance and stable across 3,144 publicly available N. gonorrhoeae genomes. Our work supports the feasibility of implementing RGT for gonorrhea into routine molecular workflows.

KW - Antimicrobial resistance

KW - Molecular diagnostic

KW - Neisseria gonorrhoeae

KW - Whole-genome sequencing

U2 - 10.1128/JCM.00089-21

DO - 10.1128/JCM.00089-21

M3 - Article

C2 - 33658263

AN - SCOPUS:85105054079

SN - 0095-1137

VL - 59

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 5

M1 - e00089-21

ER -

Reflex detection of ciprofloxacin resistance in Neisseria gonorrhoeae by use of the SpeeDx ResistancePlus GC assay

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Keywords

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