Reduced incorporation of the influenza B virus BM2 protein in virus particles decreases infectivity

David Jackson, T Zurcher, W Barclay

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

BM2 is the fourth integral membrane protein encoded by the influenza B virus genome. It is synthesized late in infection and transported to the plasma membrane from where it is subsequently incorporated into progeny virus particles. It has recently been reported that BM2 has ion channel activity and may be the functional homologue of the influenza A virus M2 protein acting as an ion channel involved in viral entry. Using a reverse genetic approach it was not possible to recover virus which lacked BM2. A recombinant influenza B virus was generated in which the BM2 AUG initiation codon was mutated to GUG. This decreased the efficiency of translation of BM2 protein such that progeny virions contained only 1/8 the amount of BM2 seen in wild-type virus. The reduction in BM2 incorporation resulted in a reduction in infectivity although there was no concomitant decrease in the numbers of virions released from the infected cells. These data imply that the incorporation of sufficient BM2 protein into influenza B virions is required for infectivity of the virus particles. (C) 2004 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)276-285
Number of pages10
JournalVirology
Volume322
Issue number2
DOIs
Publication statusPublished - 1 May 2004

Keywords

  • BM2
  • influenza B virus
  • virus
  • ION-CHANNEL ACTIVITY
  • INTEGRAL MEMBRANE-PROTEIN
  • BICISTRONIC MESSENGER-RNA
  • OPEN READING FRAME
  • A VIRUS
  • M(2) PROTEIN
  • M2 PROTEIN
  • NB PROTEIN
  • ACTIVE FORM
  • GUG CODON

Fingerprint

Dive into the research topics of 'Reduced incorporation of the influenza B virus BM2 protein in virus particles decreases infectivity'. Together they form a unique fingerprint.

Cite this