Recombinant human PPAR-beta/delta ligand-binding domain is locked in an activated conformation by endogenous fatty acids

S A Fyffe, M S Alphey, L Buetow, Terry K Smith, M A J Ferguson, M D Sorensen, F Bjorkling, W N Hunter

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

High-resolution crystallographic structures of recombinant human peroxisome proliferator-activated receptor ligand-binding domain (isotype beta/delta) reveal a fatty acid in the binding site. Mass spectrometry confirmed the presence of C16:0, C16:1, C18:0 and C18:1 in a ratio of approximately 3:2:1:4 with 11, Z-octadecenoic acid (cis-vaccenic acid) identified as the predominant species. These are endogenous fatty acids acquired from the bacterial expression system, and serve to lock the ligand-binding domain into the activated conformation. A requirement for crystal growth, the additive n-heptyl-beta-(D)-glucopyranoside, binds near the activation function helix where recognition of co-activator proteins occurs. Our observations suggest potential physiological ligands for human PPAR-beta/delta and highlight that reported binding studies must be treated with caution unless endogenous fatty acids have been removed from the sample prior to analysis. (c) 2005 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1005-1013
Number of pages9
JournalJournal of Molecular Biology
Volume356
Issue number4
DOIs
Publication statusPublished - 3 Mar 2006

Keywords

  • crystal structure
  • fatty acid
  • ligand-binding domain
  • mass spectrometry
  • nuclear receptor
  • CRYSTAL-STRUCTURE
  • NUCLEAR RECEPTOR
  • ORPHAN RECEPTORS
  • COMPLEX
  • ALPHA
  • DELTA
  • GAMMA

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