Recognition of immunogold markers in electron micrographs

Ruixuan Wang, Himanshu Pokhariya, Stephen J. McKenna, John Milton Lucocq

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Immunoelectron microscopy is used in cell biological research to study the spatial distribution of intracellular macromolecules at the ultrastructural level. Colloidal gold particles (immunogold markers) are commonly used to localise molecules of interest on ultrathin sections and can be visualised in transmission electron micrographs as dark spots. Quantitative analysis involves detection of the immunogold markers, and is often performed manually or interactively as part of a stereological estimation technique. The method presented in this paper automatically detects and counts immunogold markers, estimating the location, size and type of each marker. It was evaluated on single-labelled as well as double-labelled images showing markers of two different sizes. This is a first step towards automatic analysis of immunoelectron micrographs, enabling a rapid and more complete quantitative analysis than is currently practicable. (C) 2011 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalJournal of Structural Biology
Volume176
Issue number2
DOIs
Publication statusPublished - Nov 2011

Keywords

  • Algorithms
  • Biological Markers
  • Cryoultramicrotomy
  • Golgi Apparatus
  • Image Processing, Computer-Assisted
  • Immunohistochemistry
  • Lysosomes
  • Microscopy, Immunoelectron
  • Reproducibility of Results

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