TY - JOUR
T1 - Recent advances in metalloproteomics
AU - Coverdale, James P. C.
AU - Polepalli, Sirilata
AU - Arruda, Marco A. Z.
AU - Santos Da Silva, Ana B.
AU - Stewart, Alan J.
AU - Blindauer, Claudia A.
N1 - Funding: This work was funded by the BBSRC (Biological and Biotechnological Sciences Research Council), Grant No BB/V014684/1. We also thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo), Grant Nos. 2014/50867-3, 2018/25207-0 and 2020/08543-7, and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnologico), Grant No 303231/2020-3. JPCC thanks the Royal Society of Chemistry for an Enablement grant (E22-1637945680). The APC was funded by MDPI.
.
PY - 2024/1/13
Y1 - 2024/1/13
N2 - Interactions between proteins and metal ions and their complexes are important in many areas of the life sciences, including physiology, medicine and toxicology. Despite the involvement of essential elements in all major processes necessary for sustaining life, metalloproteomes remain ill-defined. This is not only owed to the complexity of metalloproteomes, but also the non-covalent character of the complexes that most essential metals form, which complicates analysis. Similar issues may also be encountered for some toxic metals. The review discusses recently developed approaches and current challenges for the study of interactions involving entire (sub-)proteomes with such labile metal ions. In the second part transition metals from the 4th and 5th period are examined, most of which are xenobiotic, and also tend to form more inert complexes. A large research area in this respect concerns metallodrug-protein interactions. Particular attention is paid to separation approaches, as these need to be adapted to the reactivity of the metal under consideration.
AB - Interactions between proteins and metal ions and their complexes are important in many areas of the life sciences, including physiology, medicine and toxicology. Despite the involvement of essential elements in all major processes necessary for sustaining life, metalloproteomes remain ill-defined. This is not only owed to the complexity of metalloproteomes, but also the non-covalent character of the complexes that most essential metals form, which complicates analysis. Similar issues may also be encountered for some toxic metals. The review discusses recently developed approaches and current challenges for the study of interactions involving entire (sub-)proteomes with such labile metal ions. In the second part transition metals from the 4th and 5th period are examined, most of which are xenobiotic, and also tend to form more inert complexes. A large research area in this respect concerns metallodrug-protein interactions. Particular attention is paid to separation approaches, as these need to be adapted to the reactivity of the metal under consideration.
KW - Metalloproteome
KW - Essential metals
KW - Xenobiotic metals
KW - Metallodrugs
KW - Ligand exchange kinetics
UR - https://www.mdpi.com/journal/biomolecules/special_issues/Biomolecule_Metal
U2 - 10.3390/biom14010104
DO - 10.3390/biom14010104
M3 - Review article
SN - 2218-273X
VL - 14
JO - Biomolecules
JF - Biomolecules
IS - 1
M1 - 104
ER -