Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform

Vincent O. Baron; Mingzhou Chen; Bjorn Hammarstrom; Robert J. H. Hammond; Peter Glynne-Jones; Stephen H. Gillespie; Kishan Dholakia

doi:10.1038/s42003-020-0915-3

Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform

Vincent O. Baron, Mingzhou Chen^*, Bjorn Hammarstrom, Robert J. H. Hammond, Peter Glynne-Jones, Stephen H. Gillespie^*, Kishan Dholakia

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Tuberculosis (TB) remains a leading cause of death worldwide. Lipid rich, phenotypically antibiotic tolerant, bacteria are more resistant to antibiotics and may be responsible for relapse and the need for long-term TB treatment. We present a microfluidic system that acoustically traps live mycobacteria, M. smegmatis, a model organism for M. tuberculosis. We then perform optical analysis in the form of wavelength modulated Raman spectroscopy (WMRS) on the trapped M. smegmatis for up to eight hours, and also in the presence of isoniazid (INH). The Raman fingerprints of M. smegmatis exposed to INH change substantially in comparison to the unstressed condition. Our work provides a real-time assessment of the impact of INH on the increase of lipids in these mycobacteria, which could render the cells more tolerant to antibiotics. This microfluidic platform may be used to study any microorganism and to dynamically monitor its response to different conditions and stimuli.

Original language	English
Article number	236
Number of pages	8
Journal	Communications Biology
Volume	3
DOIs	https://doi.org/10.1038/s42003-020-0915-3
Publication status	Published - 14 May 2020

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Baron_2020_CB_Real-time_CC
Copyright © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Final published version, 1.07 MBLicence: CC BY

Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world
Dholakia, K. (PI) & Gather, M. C. (CoI)
1/08/17 → 31/07/22
Project: Standard
IMI PreDiCT-TB - Physics: IMI Pre-DiCT-TB
Dholakia, K. (PI)
European Commission
1/05/12 → 31/10/17
Project: Standard
IMI PREDICT TB: EU Innovative Medicines Initiative (IMI) 75% account
Gillespie, S. H. (PI)
European Commission
1/05/12 → 31/10/17
Project: Standard

Robert Hammond
- rjhhst-andrews.acuk
- School of Medicine - Lecturer
- Infection and Global Health Division
Person: Academic

Data underpinning: Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform
Baron, V. O. (Creator), Chen, M. (Creator), Hammarstrom, B. (Creator), Hammond, R. J. H. (Creator), Glynne-Jones, P. (Creator), Gillespie, S. H. (Creator) & Dholakia, K. (Creator), University of St Andrews, 25 Mar 2020
DOI: 10.17630/8fd4d829-b63b-4e72-a14e-921eee1638a7
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File

Cite this

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title = "Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform",

abstract = "Tuberculosis (TB) remains a leading cause of death worldwide. Lipid rich, phenotypically antibiotic tolerant, bacteria are more resistant to antibiotics and may be responsible for relapse and the need for long-term TB treatment. We present a microfluidic system that acoustically traps live mycobacteria, M. smegmatis, a model organism for M. tuberculosis. We then perform optical analysis in the form of wavelength modulated Raman spectroscopy (WMRS) on the trapped M. smegmatis for up to eight hours, and also in the presence of isoniazid (INH). The Raman fingerprints of M. smegmatis exposed to INH change substantially in comparison to the unstressed condition. Our work provides a real-time assessment of the impact of INH on the increase of lipids in these mycobacteria, which could render the cells more tolerant to antibiotics. This microfluidic platform may be used to study any microorganism and to dynamically monitor its response to different conditions and stimuli.",

author = "Baron, {Vincent O.} and Mingzhou Chen and Bjorn Hammarstrom and Hammond, {Robert J. H.} and Peter Glynne-Jones and Gillespie, {Stephen H.} and Kishan Dholakia",

note = "Funding: UK Engineering and Physical Sciences Research Council for funding through grant EP/P030017/1 and fellowship EP/L025035/1. This work was also supported by the PreDiCT-TB consortium [IMI Joint undertaking grant agreement number 115337, resources of which are composed of financial contribution from the European Union{\textquoteright}s Seventh Framework Programme (FP7/2007–2013) and EFPIA companies{\textquoteright} in kind contribution (www.imi.europa.eu)] and the PanACEA consortium [funded by the European & Developing Countries Clinical Trials Partnership (EDCTP); grant agreement: TRIA-2015-1102]",

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doi = "10.1038/s42003-020-0915-3",

language = "English",

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T1 - Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform

AU - Baron, Vincent O.

AU - Chen, Mingzhou

AU - Hammarstrom, Bjorn

AU - Hammond, Robert J. H.

AU - Glynne-Jones, Peter

AU - Gillespie, Stephen H.

AU - Dholakia, Kishan

N1 - Funding: UK Engineering and Physical Sciences Research Council for funding through grant EP/P030017/1 and fellowship EP/L025035/1. This work was also supported by the PreDiCT-TB consortium [IMI Joint undertaking grant agreement number 115337, resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007–2013) and EFPIA companies’ in kind contribution (www.imi.europa.eu)] and the PanACEA consortium [funded by the European & Developing Countries Clinical Trials Partnership (EDCTP); grant agreement: TRIA-2015-1102]

PY - 2020/5/14

Y1 - 2020/5/14

N2 - Tuberculosis (TB) remains a leading cause of death worldwide. Lipid rich, phenotypically antibiotic tolerant, bacteria are more resistant to antibiotics and may be responsible for relapse and the need for long-term TB treatment. We present a microfluidic system that acoustically traps live mycobacteria, M. smegmatis, a model organism for M. tuberculosis. We then perform optical analysis in the form of wavelength modulated Raman spectroscopy (WMRS) on the trapped M. smegmatis for up to eight hours, and also in the presence of isoniazid (INH). The Raman fingerprints of M. smegmatis exposed to INH change substantially in comparison to the unstressed condition. Our work provides a real-time assessment of the impact of INH on the increase of lipids in these mycobacteria, which could render the cells more tolerant to antibiotics. This microfluidic platform may be used to study any microorganism and to dynamically monitor its response to different conditions and stimuli.

AB - Tuberculosis (TB) remains a leading cause of death worldwide. Lipid rich, phenotypically antibiotic tolerant, bacteria are more resistant to antibiotics and may be responsible for relapse and the need for long-term TB treatment. We present a microfluidic system that acoustically traps live mycobacteria, M. smegmatis, a model organism for M. tuberculosis. We then perform optical analysis in the form of wavelength modulated Raman spectroscopy (WMRS) on the trapped M. smegmatis for up to eight hours, and also in the presence of isoniazid (INH). The Raman fingerprints of M. smegmatis exposed to INH change substantially in comparison to the unstressed condition. Our work provides a real-time assessment of the impact of INH on the increase of lipids in these mycobacteria, which could render the cells more tolerant to antibiotics. This microfluidic platform may be used to study any microorganism and to dynamically monitor its response to different conditions and stimuli.

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DO - 10.1038/s42003-020-0915-3

M3 - Article

SN - 2399-3642

VL - 3

JO - Communications Biology

JF - Communications Biology

M1 - 236

ER -

Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform

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Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world

IMI PreDiCT-TB - Physics: IMI Pre-DiCT-TB

IMI PREDICT TB: EU Innovative Medicines Initiative (IMI) 75% account

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Robert Hammond

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Data underpinning: Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform

Cite this