Read-through protein gene of potato mop-top furovirus is associated with acquisition and transmission of the virus by Spongospora Subterranea F.SP. Subterranea

Mohammed Arif*, Brian Reavy, Lesley Torrance

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Spontaneous deletion was detected in read-through domain of potato mop-top furovirus (PMTV) RNA 3 of isolate T by reverse transcription-polymerase chain reaction (RT-PCR). PCR amplification of read-through of PMTV-T and comparison with PMTV-S, a full-length isolate, indicated 700 nucleotides difference in size but no difference in coat protein gene. Sequence analysis of the 5′-end of the read-through gene of PMTV-T relative to that of PMTV-S occurs in 3′-half of the gene and is associated with loss of transmission by the fungus vector, Spongospora subterranea f. sp. subterranea. A mono-fungal S. subterranea f. sp. subterranea was unable to acquire and transmit PMTV-T which had 700 nucleotides deletion in 5′-half of read-through domain of PMTV RNA 3, while PMTV-S with full-length read-through gene was efficiently acquired and transmitted by the same fungus cultures. These results indicated that read-through gene encoded on RNA 3 is involved in acquisition and transmission of PMTV by its fungal vector, S. subterranea f. sp. subterranea.

Original languageEnglish
Pages (from-to)125-136
Number of pages12
JournalPakistan Journal of Botany
Volume31
Issue number1
Publication statusPublished - 1 Jun 1999

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