@article{0e77869d56474fdca54cc8366ac5ac39,
title = "Rapid single-colony whole-genome sequencing of bacterial pathogens",
abstract = "As a result of the introduction of rapid benchtop sequencers, the time required to subculture a bacterial pathogen to extract sufficient DNA for library preparation can now exceed the time to sequence said DNA. We have eliminated this rate-limiting step by developing a protocol to generate DNA libraries for whole-genome sequencing directly from single bacterial colonies grown on primary culture plates.",
author = "K{\"o}ser, \{Claudio U\} and Fraser, \{Louise J\} and Avgousta Ioannou and Jennifer Becq and Ellington, \{Matthew J\} and Holden, \{Matthew T G\} and Sandra Reuter and T{\"o}r{\"o}k, \{M Est{\'e}e\} and Bentley, \{Stephen D\} and Julian Parkhill and Gormley, \{Niall A\} and Smith, \{Geoffrey P\} and Peacock, \{Sharon J\}",
year = "2013",
month = dec,
day = "25",
doi = "10.1093/jac/dkt494",
language = "English",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
}