Rapid single-colony whole-genome sequencing of bacterial pathogens

Claudio U Köser, Louise J Fraser, Avgousta Ioannou, Jennifer Becq, Matthew J Ellington, Matthew T G Holden, Sandra Reuter, M Estée Török, Stephen D Bentley, Julian Parkhill, Niall A Gormley, Geoffrey P Smith, Sharon J Peacock

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

As a result of the introduction of rapid benchtop sequencers, the time required to subculture a bacterial pathogen to extract sufficient DNA for library preparation can now exceed the time to sequence said DNA. We have eliminated this rate-limiting step by developing a protocol to generate DNA libraries for whole-genome sequencing directly from single bacterial colonies grown on primary culture plates.
Original languageEnglish
JournalJournal of Antimicrobial Chemotherapy
DOIs
Publication statusPublished - 25 Dec 2013

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