Rapid single-colony whole-genome sequencing of bacterial pathogens

Claudio U Köser; Louise J Fraser; Avgousta Ioannou; Jennifer Becq; Matthew J Ellington; Matthew T G Holden; Sandra Reuter; M Estée Török; Stephen D Bentley; Julian Parkhill; Niall A Gormley; Geoffrey P Smith; Sharon J Peacock

doi:10.1093/jac/dkt494

Rapid single-colony whole-genome sequencing of bacterial pathogens

Claudio U Köser, Louise J Fraser, Avgousta Ioannou, Jennifer Becq, Matthew J Ellington, Matthew T G Holden, Sandra Reuter, M Estée Török, Stephen D Bentley, Julian Parkhill, Niall A Gormley, Geoffrey P Smith, Sharon J Peacock

Research output: Contribution to journal › Article › peer-review

Abstract

As a result of the introduction of rapid benchtop sequencers, the time required to subculture a bacterial pathogen to extract sufficient DNA for library preparation can now exceed the time to sequence said DNA. We have eliminated this rate-limiting step by developing a protocol to generate DNA libraries for whole-genome sequencing directly from single bacterial colonies grown on primary culture plates.

Original language	English
Journal	Journal of Antimicrobial Chemotherapy
DOIs	https://doi.org/10.1093/jac/dkt494
Publication status	Published - 25 Dec 2013

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10.1093/jac/dkt494

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title = "Rapid single-colony whole-genome sequencing of bacterial pathogens",

abstract = "As a result of the introduction of rapid benchtop sequencers, the time required to subculture a bacterial pathogen to extract sufficient DNA for library preparation can now exceed the time to sequence said DNA. We have eliminated this rate-limiting step by developing a protocol to generate DNA libraries for whole-genome sequencing directly from single bacterial colonies grown on primary culture plates.",

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AU - Köser, Claudio U

AU - Fraser, Louise J

AU - Ioannou, Avgousta

AU - Becq, Jennifer

AU - Ellington, Matthew J

AU - Holden, Matthew T G

AU - Reuter, Sandra

AU - Török, M Estée

AU - Bentley, Stephen D

AU - Parkhill, Julian

AU - Gormley, Niall A

AU - Smith, Geoffrey P

AU - Peacock, Sharon J

PY - 2013/12/25

Y1 - 2013/12/25

N2 - As a result of the introduction of rapid benchtop sequencers, the time required to subculture a bacterial pathogen to extract sufficient DNA for library preparation can now exceed the time to sequence said DNA. We have eliminated this rate-limiting step by developing a protocol to generate DNA libraries for whole-genome sequencing directly from single bacterial colonies grown on primary culture plates.

AB - As a result of the introduction of rapid benchtop sequencers, the time required to subculture a bacterial pathogen to extract sufficient DNA for library preparation can now exceed the time to sequence said DNA. We have eliminated this rate-limiting step by developing a protocol to generate DNA libraries for whole-genome sequencing directly from single bacterial colonies grown on primary culture plates.

U2 - 10.1093/jac/dkt494

DO - 10.1093/jac/dkt494

M3 - Article

C2 - 24370932

SN - 0305-7453

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

ER -

Rapid single-colony whole-genome sequencing of bacterial pathogens

Abstract

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