Rapid gene discovery in plant parasitic nematodes via Expressed Sequence Tags

J McCarter; P Abad; JT Jones; D Bird

Rapid gene discovery in plant parasitic nematodes via Expressed Sequence Tags

J McCarter, P Abad, JT Jones, D Bird^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Projects currently underway are generating thousands of publicly available DNA sequences representing numerous genes from plant parasitic nematodes. Use of these data has the potential to revolutionise gene discovery, as well as aiding in genome physical mapping and expression profiling experiments. This article introduces sequences called expressed sequence tags or ESTs, which are single-sequence reads from randomly-selected cDNA clones. We review the process used to create these sequences and outline the strengths and weaknesses of ESTs as research tools. Instructions on how to access and use EST data also are provided.

Original language	English
Pages (from-to)	719-731
Number of pages	13
Journal	Nematology
Volume	2
Publication status	Published - 2000

Keywords

Caenorhabditis elegans
clustering
EST
Globodera
Meloidogyne
NemaGene
CAENORHABDITIS-ELEGANS
ARABIDOPSIS-THALIANA
BRUGIA-MALAYI
HUMAN GENOME
C-ELEGANS
HOMOLOGS
BRIGGSAE
CLONING
DNA
BETA-1,4-ENDOGLUCANASE

Cite this

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title = "Rapid gene discovery in plant parasitic nematodes via Expressed Sequence Tags",

abstract = "Projects currently underway are generating thousands of publicly available DNA sequences representing numerous genes from plant parasitic nematodes. Use of these data has the potential to revolutionise gene discovery, as well as aiding in genome physical mapping and expression profiling experiments. This article introduces sequences called expressed sequence tags or ESTs, which are single-sequence reads from randomly-selected cDNA clones. We review the process used to create these sequences and outline the strengths and weaknesses of ESTs as research tools. Instructions on how to access and use EST data also are provided.",

keywords = "Caenorhabditis elegans, clustering, EST, Globodera, Meloidogyne, NemaGene, CAENORHABDITIS-ELEGANS, ARABIDOPSIS-THALIANA, BRUGIA-MALAYI, HUMAN GENOME, C-ELEGANS, HOMOLOGS, BRIGGSAE, CLONING, DNA, BETA-1,4-ENDOGLUCANASE",

author = "J McCarter and P Abad and JT Jones and D Bird",

year = "2000",

language = "English",

volume = "2",

pages = "719--731",

journal = "Nematology",

issn = "1388-5545",

publisher = "Brill",

}

TY - JOUR

T1 - Rapid gene discovery in plant parasitic nematodes via Expressed Sequence Tags

AU - McCarter, J

AU - Abad, P

AU - Jones, JT

AU - Bird, D

PY - 2000

Y1 - 2000

N2 - Projects currently underway are generating thousands of publicly available DNA sequences representing numerous genes from plant parasitic nematodes. Use of these data has the potential to revolutionise gene discovery, as well as aiding in genome physical mapping and expression profiling experiments. This article introduces sequences called expressed sequence tags or ESTs, which are single-sequence reads from randomly-selected cDNA clones. We review the process used to create these sequences and outline the strengths and weaknesses of ESTs as research tools. Instructions on how to access and use EST data also are provided.

AB - Projects currently underway are generating thousands of publicly available DNA sequences representing numerous genes from plant parasitic nematodes. Use of these data has the potential to revolutionise gene discovery, as well as aiding in genome physical mapping and expression profiling experiments. This article introduces sequences called expressed sequence tags or ESTs, which are single-sequence reads from randomly-selected cDNA clones. We review the process used to create these sequences and outline the strengths and weaknesses of ESTs as research tools. Instructions on how to access and use EST data also are provided.

KW - Caenorhabditis elegans

KW - clustering

KW - EST

KW - Globodera

KW - Meloidogyne

KW - NemaGene

KW - CAENORHABDITIS-ELEGANS

KW - ARABIDOPSIS-THALIANA

KW - BRUGIA-MALAYI

KW - HUMAN GENOME

KW - C-ELEGANS

KW - HOMOLOGS

KW - BRIGGSAE

KW - CLONING

KW - DNA

KW - BETA-1,4-ENDOGLUCANASE

M3 - Article

SN - 1388-5545

VL - 2

SP - 719

EP - 731

JO - Nematology

JF - Nematology

ER -

Rapid gene discovery in plant parasitic nematodes via Expressed Sequence Tags

Abstract

Keywords

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