Rapid gene discovery in plant parasitic nematodes via Expressed Sequence Tags

J McCarter, P Abad, JT Jones, D Bird*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

Projects currently underway are generating thousands of publicly available DNA sequences representing numerous genes from plant parasitic nematodes. Use of these data has the potential to revolutionise gene discovery, as well as aiding in genome physical mapping and expression profiling experiments. This article introduces sequences called expressed sequence tags or ESTs, which are single-sequence reads from randomly-selected cDNA clones. We review the process used to create these sequences and outline the strengths and weaknesses of ESTs as research tools. Instructions on how to access and use EST data also are provided.

Original languageEnglish
Pages (from-to)719-731
Number of pages13
JournalNematology
Volume2
Publication statusPublished - 2000

Keywords

  • Caenorhabditis elegans
  • clustering
  • EST
  • Globodera
  • Meloidogyne
  • NemaGene
  • CAENORHABDITIS-ELEGANS
  • ARABIDOPSIS-THALIANA
  • BRUGIA-MALAYI
  • HUMAN GENOME
  • C-ELEGANS
  • HOMOLOGS
  • BRIGGSAE
  • CLONING
  • DNA
  • BETA-1,4-ENDOGLUCANASE

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