Rapid diagnosis of whooping cough using monoclonal antibody

P. C. Boreland; S. H. Gillespie; L. A.E. Ashworth

doi:10.1136/jcp.41.5.573

Rapid diagnosis of whooping cough using monoclonal antibody

P. C. Boreland, S. H. Gillespie, L. A.E. Ashworth

Research output: Contribution to journal › Article › peer-review

Abstract

A counterimmunoelectrophoresis (CIE) method for antigen detection using monoclonal antibody was assessed for its ability to aid in the rapid diagnosis of Bordetella pertussis in 59 patients. A positive diagnosis from a combination of results from tests of serum and urine was obtained in 51 (87%) of cases. For sera, CIE had a sensitivity of 85% and a specificity of 94%; for urine samples the sensitivity was 81% and a specificity of 100%. Antigen detection by CIE is simple to perform and yields results on the same day, thus allowing treatment to begin at an early stage.

Original language	English
Pages (from-to)	573-575
Number of pages	3
Journal	Journal of Clinical Pathology
Volume	41
Issue number	5
DOIs	https://doi.org/10.1136/jcp.41.5.573
Publication status	Published - 1 Jan 1988

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title = "Rapid diagnosis of whooping cough using monoclonal antibody",

abstract = "A counterimmunoelectrophoresis (CIE) method for antigen detection using monoclonal antibody was assessed for its ability to aid in the rapid diagnosis of Bordetella pertussis in 59 patients. A positive diagnosis from a combination of results from tests of serum and urine was obtained in 51 (87\%) of cases. For sera, CIE had a sensitivity of 85\% and a specificity of 94\%; for urine samples the sensitivity was 81\% and a specificity of 100\%. Antigen detection by CIE is simple to perform and yields results on the same day, thus allowing treatment to begin at an early stage.",

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year = "1988",

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AU - Boreland, P. C.

AU - Gillespie, S. H.

AU - Ashworth, L. A.E.

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N2 - A counterimmunoelectrophoresis (CIE) method for antigen detection using monoclonal antibody was assessed for its ability to aid in the rapid diagnosis of Bordetella pertussis in 59 patients. A positive diagnosis from a combination of results from tests of serum and urine was obtained in 51 (87%) of cases. For sera, CIE had a sensitivity of 85% and a specificity of 94%; for urine samples the sensitivity was 81% and a specificity of 100%. Antigen detection by CIE is simple to perform and yields results on the same day, thus allowing treatment to begin at an early stage.

AB - A counterimmunoelectrophoresis (CIE) method for antigen detection using monoclonal antibody was assessed for its ability to aid in the rapid diagnosis of Bordetella pertussis in 59 patients. A positive diagnosis from a combination of results from tests of serum and urine was obtained in 51 (87%) of cases. For sera, CIE had a sensitivity of 85% and a specificity of 94%; for urine samples the sensitivity was 81% and a specificity of 100%. Antigen detection by CIE is simple to perform and yields results on the same day, thus allowing treatment to begin at an early stage.

UR - https://www.scopus.com/pages/publications/0023884836

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SP - 573

EP - 575

JO - Journal of Clinical Pathology

JF - Journal of Clinical Pathology

IS - 5

ER -

Rapid diagnosis of whooping cough using monoclonal antibody

Abstract

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