Abstract
The technique of rapid acidification and alkylation can be used to characterise the redox status of oxidoreductases, and to determine numbers of free cysteine residues within substrate proteins. We have previously used this method to analyse interacting components of the MHC class I pathway, namely ERp57 and tapasin. Here, we have applied rapid acidification alkylation as a novel approach to analysing the redox status of MHC class I molecules. This analysis of the redox status of the MHC class I molecules HLA-A2 and HLA-B27, which is strongly associated with a group of inflammatory arthritic disorders referred to as Spondyloarthropathies, revealed structural and conformational information. We propose that this assay provides a useful tool in the study of in vivo MHC class I structure. (c) 2008 Elsevier B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 81-85 |
Number of pages | 5 |
Journal | Journal of Immunological Methods |
Volume | 340 |
Issue number | 1 |
Early online date | 7 Oct 2008 |
DOIs | |
Publication status | Published - 1 Jan 2009 |
Keywords
- MHC class I
- Redox
- Alkylation
- Oxidoreductases
- MAJOR HISTOCOMPATIBILITY COMPLEX
- PEPTIDE-LOADING COMPLEX
- TRANSGENIC RATS
- ERP57
- HLA-B27